Histology Stains

Mast Cell Stain (Toluidine blue method) Baso Clear Colourful Stains What is 'Mast Cell Stain (Toluidine blue method)'Mast cell particles contain heparin and histamine etc. These are sulfuric acid esters, are heterochromatic, so with the metachromatic dye toluidine blue staining, it can be appeared heterochromatic purplish-red. Stain ResultMast cell granulespurplish-redcell nuclearblue The Main ComponentsReagent compositionMain ingredient1. Toluidine blue solutionToluidine

Calcium Salt stain (Silver Nitrate Method) Baso Clear Colourful Stains Testing Principle It is a metal replacement method. When silver nitrate solution acting on the insoluble calcium salts of histological section, calcium salt is converted to silver. Silver salt will be reduced to the black metallic silver because of the effect of light (such as sunlight or ultraviolet light). Stain Results Calcium salt appears brown black to deep black. Packaging Specification Product Name

Masson's Trichrome Stain (Celestin blue method) Baso Clear Colourful Stains What is Masson's Trichrome Stain (Celestin blue method) This staining solution is an improved version of Mallory's trichrome staining method, utilizing a mixture of two or three anionic dyes applied either simultaneously or sequentially to complete the staining process. The staining mechanism is related to the molecular size of the anionic dyes and the permeability of the tissues. By selecting anionic

Masson's Trichrome Stain (Weigert's iron hematoxylin method) Baso Clear Colourful Stains Testing Principle This method is modified from Mallory's Trichome stain, which uses the mixture of two or three anion dyes or itsfunction in sequence to stain the specimen. Staining results can be affected by the molecular weight of aniondyes and tissue permeability. Demonstration of different tissue components can be made from selection of aniondyes with different molecular weight and

Testing Principle N,N-Dimethyl-m-phenylenediamine dihydrochloride and N,N-dimethyl-p-phenylenediamine dihydrochloride are both amine salts that dissociate to carry positive charges. The diamine salts combine with sulfated acidic mucosubstances to form colored complexes. This reaction is slow and requires ferric chloride (FeCl₃) as a catalyst. On one hand, FeCl₃ oxidizes the diamine salts to form brown-black cationic chromogens, thereby accelerating the staining process. On

Colloidal Iron Stain Baso Clear Colourful Stains for Healthcare Facility Testing Principle Under acidic conditions, acidic mucopolysaccharides can adsorb Fe³⁺ from colloidal iron staining solution to form a stable polymer. This polymer then reacts with potassium ferrocyanide and hydrochloric acid to produce a Prussian blue reaction, resulting in a blue color. Testiing Results Acidic mucopolysaccharides appear blue, while other tissue cells appear light red. Packaging

Testing Principle Alcian blue is the most specific stain to detect acid mucous substances. This anion dye forms an electrovalent bond with acid group. Alcian blue can be used to separate and identify different mucous substances at various pH. At pH 1, Carboxyl group (COOH) was not stained, sulfonic group (OSO 3 H) is stained; and at pH 2.5, carboxyl group is well stained, but poor stain of sulfate mucus. Stain Results Sialic acid and weakly sulfated mucilage Blue General

Testing Principle Alcian blue is the most specific stain to detect acid mucous substances. This anion dye forms an electrovalent bond with acid group. Alcian blue can be used to separate and identify different mucous substances at various pH. At pH 1, Carboxyl group (COOH) was not stained, sulfonic group (OSO 3 H) is stained; and at pH 2.5, carboxyl group is well stained, but poor stain of sulfate mucus. Stain Results Contain sulfated mucilage substance Blue Non-sulfated acid

Testing Principle Neutral mucus contains amino hexose and free hexose. Acid mucus also contains amino hexose and different kinds of acid radical. Neutral mucus is found in gastric mucosa, duodenum gland, submandibular gland and prostatic epithelium. Acid mucus is found in respiratory tract, goblet cells of digestive tract, aortic wall, cartilage matrix, cornea and skin. Gastric mucosa secretes neutral mucus. Acid mucus is secreted in intestinal metaplasia of stomach or

Testing Principle Neutral mucus contains amino hexose and free hexose. Acid mucus also contains amino hexose and different kinds of acid radical. Neutral mucus is found in gastric mucosa, duodenum gland, submandibular gland and prostatic epithelium. Acid mucus is found in respiratory tract, goblet cells of digestive tract, aortic wall, cartilage matrix, cornea and skin. Gastric mucosa secretes neutral mucus. Acid mucus is secreted in intestinal metaplasia of stomach or

Testing Principle Amylase is a generic term for enzymes that hydrolyze starch and glycogen. Amylase generally acts on soluble starch, amylose, glycogen, etc., and cuts off the α -1,4-chain indiscriminately, and decomposes it into disaccharides and monosaccharides. Since monosaccharides are extracted during histochemical operations such as immobilization, dehydration, and embedding, the sugars that can be displayed on general tissue specimens are mainly polysaccharides,

Testing Principle Periodic acid is an oxidant which can destroy carbon bond of polyose. First, tissues are oxidized in periodic acid solution to break open the carbon bond of glycol group or ammonia hydroxyl in tissue polyose, which results in the formation of aldehyde compound. Subsequently, the free aldehyde exposed will react with colorless Fuchsin to form a new red- fuchsia compound. Stain Results Mold, Glycogen, Neutral mucus red Nuclei blue Packaging Specification

Testing Principle Periodic acid is an oxidant which can destroy carbon bond of polyose. First, tissues are oxidized in periodic acid solution to break open the carbon bond of glycol group or ammonia hydroxyl in tissue polyose, which results in the formation of aldehyde compound. Subsequently, the free aldehyde exposed will react with colorless Fuchsin to form a new red- fuchsia compound. Stain Results Mold, Glycogen, Neutral mucus red Nuclei blue Packaging Specification

Testing Principle Alkaline fuchsin is mixed with paraformaldehyde. Under the catalysis of hydrochloric acid, paraformaldehyde gradually depolymerizes to release acetaldehyde. The acetaldehyde reacts with the amino groups of the alkaline fuchsia dye to form a deep purple acetaldehyde-alkaline fuchsia complex, known as aldehyde fuchsin. During this process, the solution gradually changes from red to purple, indicating its maturation, and is referred to as aldehyde fuchsin

Testing Principle The principle of this method is not well-known. Hypothesis includes that hydrogen bond forms between certain parts of elastic fiber and phenolic group of resorcinol in Elastin Stain, consequently the elastic fiber is stained blue-black. VG stain was used for contrast stain. Stain Results V.G. counterstaining Nuclear Fast Red counterstaining Elastic fiber Blue-black Blue-black Collagen fiber Red Red Muscle fiber, Erythrocyte Yellow Red Packaging Specification

Testing Principle The principle of this method is not well-known. Hypothesis includes that hydrogen bond forms between certain parts of elastic fiber and phenolic group of resorcinol in Elastin Stain, consequently the elastic fiber is stained blue-black. VG stain was used for contrast stain. Stain results V.G. counterstaining Nuclear Fast Red counterstaining Elastic fiber Blue-black Blue-black Collagen fiber Red Red Muscle fiber, Erythrocyte Yellow Red Packaging Specification

Testing Principle This method mainly uses acid fuchsin and picric acid to double dye the collagen fiber and the muscle fiber respectively. The bitter acid has a small molecular weight, can penetrate muscle fibers, glial cells and red blood cells with dense tissue structure combining with bitter acid were stained in yellow; The acid fuchsin has a medium to large molecular weight, and can only penetrate collagen fibers with looser tissue structure. combining with acid fuchsin

Testing Principle Hemosiderin is a hemoglobin-derived pigment that is golden yellow or yellowish brown in size and shape. Becauseit contains iron, it is called hemosiderin. The iron in hemosiderin is generally ferric iron or divalent iron, and ismainly composed of trivalent iron. The principle that the use of ferric iron can be separated from the protein bvdilute hydrochloric acid, and the Prussian blue reaction with potassium ferrocyanide can be used to indirectly provethe

Testing Principle Carmine can bind mordant aluminum to form a complex, which in turn binds to the acid group of mucus and becomes red. Stain Results Acidic mucin substances red to rose red nuclei purple blue other tissue components light yellow Packaging Specification Product Name Ref # Specification Mucicarmine Stain BA4086A 4x20ml Mucicarmine Stain BA4086B 4x100ml

Testing Principle This product utilizes immunofluorescence technology. After binding with the corresponding antigen in the sample, it produces specific imaging under the excitation light of a specific wavelength range in a fluorescence microscope. Under the B-band (violet-blue excitation filter, excitation wavelength: 350nm~490nm), cellular, bacterial, and other components can be observed. Stain Results Observe the morphology of fluorescent cells and pathogenic bacteria, and