DdPCR QPCR And MNGS Library Preparation Host Removal Kit In Swabs

Host Removal Kit for ddPCR, qPCR and mNGS Library Preparation in Swabs

Introduction

This kit uses magnetic beads and buffer system with separation function, which can separate and purify high quality nucleic acid from samples. Special coated magnetic beads have a strong affinity for nucleic acid in samples under certain conditions. When the conditions change, magnetic beads release the absorbed nucleic acid, so as to achieve rapid extraction and purification of nucleic acid in samples..

Samples available for extraction
• This kit is suitable for the extraction and purification of nucleic acids (DNA and RNA) in serum, plasma, virus culture, swab lotion and other samples. Samples should be stored in time after collection to avoid cross-contamination between samples.
• This kit is only used to extract nucleic acid from the sample. The sample types and storage conditions are different. For specific sample requirements, please strictly follow the relevant requirements of nucleic acid testing kit to the samples.
• Before nucleic acid extraction, the sample should be fully mixed to avoid affecting the quantity of nucleic acid extracted.
• When using this kit to extract other samples, please contact our technical service for relevant information.

Product Advantages

Various Sample Types: Widely support blood, saliva, alveolar lavage fluid, sputum, swabs and other biological fluid samples.

Unbiased: Unbiased microbial nucleic acid extraction, compatible with multiple pathogen types, minimizes microbial loss in samples.

High Efficiency and Simplicity: Fast and simple operation to minimize the host background and enrich microorganisms efficiently.

Wide Applications: Compatible with a variety of downstream applications, including PCR, qPCR, mNGS, etc., ensuring the accuracy and precision of downstream experiments.

Product Information

Company Information

BunnyTeeth Technology Inc. is committed to building a complete core technology platform for bioengineering, building a high-quality biotech product chain and a technical service chain, with the ambition to become a global leader in molecular and genetic diagnostic. BunnyTeeth Technology Inc. will also provide customers with a full-range of one-step service solution in the field of molecular diagnostics and genetic diagnostic.

BunnyTeeth Technology Inc. uses a unique unbiased lysis technology that can specifically remove host cells and nucleic acids while retaining intact bacteria, fungi, DNA viruses, chlamydia, mycoplasma and other pathogenic microorganisms in the sample. The enriched microorganisms can be used for various downstream applications such as PCR, qPCR, mNGS after DNA/RNA extraction.

Experimental Data

High quality of Microbial DNA Enrichment

Figure 1. Efficient recovery of microbial DNA enrichment.

Use Host Removal Kit for ddPCR, qPCR and mNGS Library Preparation in Swabs and Zymo similar product to remove host DNA and then extract microbial DNA from 5 body fluid samples. Compared to no host removal, BunnyPure Host Removal Kit was highly efficient at removing host DNA, and the extraction yield of BunnyPure kit was consistent with the performance of those of competing products.

Stable, balanced and accurate sequence information

Figure 3. Excellent QC data for mNGS.

Use Host Removal Kit for ddPCR, qPCR and mNGS Library Preparation in Swabs to remove host DNA in alveolar lavage samples. Microbial DNA obtained with BunnyMag Body Fluids Microbiome DNA Isolation Kit entered the mNGS process with excellent quality control performance.

Main Composition

Host Lysis Solution

Nuclease

Nuclease Buffer

Proteinase K

Storage Condition

Nuclease is stored at -20℃, Proteinase K at 2-8℃, and other reagents at room temperature.

The validity period is 12 months.

Operating Process