OEM Cell Free DNA BCT for Non-Invasive Pre-Natal Screening

OEM Cell Free DNA BCT for Non-Invasive Pre-Natal Screening

Company info

BunnyTeeth Technology Inc. is committed to building a complete core technology platform for bioengineering, building a high-quality biotech product chain and a technical service chain, with the ambition to become a global leader in molecular and genetic diagnostic. BunnyTeeth Technology Inc. will also provide customers with a full-range of one-step service solution in the field of molecular diagnostics and genetic diagnostic.

Product abstract

roduct description:

The role of plasma cell-free DNA (cfDNA) is becoming increasingly evident as an important test sample for tumor diagnosis and non-invasive prenatal screening. BunnyTube OEM Cell Free DNA BCT for Non-Invasive Pre-Natal Screening can be directly used for whole blood collection, preservation and transportation, which can effectively protect cfDNA from degradation by nucleases and avoid the release of genomic DNA from blood nucleated cells, greatly ensuring the downstream application of cfDNA.

BunnyTube OEM Cell Free DNA BCT for Non-Invasive Pre-Natal Screening contain a special stabilizer that immobilizes blood cells after collection to prevent the release of genomic DNA and inhibit the degradation of cfDNA by nucleases to maintain the overall stability of cell-free DNA. It is used for the collection, transport and storage of cell-free DNA from blood samples for clinical testing.

BunnyTube OEM Cell Free DNA BCT for Non-Invasive Pre-Natal Screening uses EDTA anticoagulant and special protective agents to stably preserve cell-free DNA in blood for up to 14 days at 6-35°C. Preserved blood samples can be used to most commercial kits for cfDNA extraction to meet the needs of research and in-vitro diagnostic applications.

Advantage

1. High Stability: Effectively avoids cfDNA degradation and genomic DNA release.
2. High Tolerance: Good temperature tolerance for easy storage and transport of samples.
3. Excellent Sequencing: The extracted cfDNA has excellent library preparation efficiency and sequencing quality.
4. Wide Application: Designed for liquid biopsies for oncology, infection, prenatal diagnosis, etc.

1. Technique:Use EDTA-K3 as anticoagulant, which has highest solubility in the liquid, to bind to Ca²⁺ ions in blood to form chelates, and inhibit thrombospondin activation to prevent blood clotting.
2. Production:Full set of reagents are manufactured in GMP production factory with ISO13485 standard; control the source production process, use scientific verification system with industry gold standard
3. Application:Thousands of clinical samples tested in the NGS testing laboratory of Geneseeq (CAP & CLIA certified), with stable results and expected performance indicators.

Figure 1. Hemolysis of blood samples and stable cfDNA yield at different storage times.

Blood samples were collected into 3 brands of preservation tubes, and the samples were placed at room temperature for 0, 3, 7 and 14 days to compare the hemolysis and cfDNA yield. BunnyTube cfDNA Preservation Tube for Body Fluid and Non-invasive Pre-natal Screening Genetic Testing CE Certified could effectively inhibit hemolysis and the extracted cfDNA yield was stable.

Figure 1. Hemolysis of blood samples and stable cfDNA yield at different transport temperatures.

The blood samples were collected into 3 brands of preservation tubes, and samples were compared for hemolysis and cfDNA yield after 0 days at room temp, 3 days at room temp (25℃), high temp (35℃) and low temp (4℃) transportation. BunnyTube cfDNA Preservation Tube for Body Fluid and Non-invasive Pre-natal Screening Genetic Testing CE Certified can withstand different transportation temperatures and have better resistance to genomic nucleic acid release

Figure 1. Excellent quality control data for NGS.

The quality control of cfDNA extracted from blood samples preserved in 产品名称 into the NGS process by our kits is excellent and shows high consistency with Streck preservation tubes.

Figure 1. Detected mutation abundance is in consistent with the theoretical abundance of the reference standard with a ratio of 1.

Simulated samples containing mutant ref. standards preserved in 产品名称 were extracted from our cfDNA kit into the NGS process, and the detected mutation abundance was highly consistent with the theoretical mutation abundance of the standards, and the mutation abundance detection showed very high similarity to Streck preservation tubes.

Component

Storage condition

Storage Temperature: 10- 30 ℃

Shelf life: 12 months

Applied Scene