Blood SuperDirect PCR Kit EDTA UNG For Dentification Of Anticoagulated Whole Blood
Blood SuperDirect PCR Kit EDTA with UNG for dentification of anticoagulated whole blood
Description
Blood SuperDirectTM PCR Kit(EDTA) -UNG uses a unique PCR buffer system, which allows direct PCR with whole blood samples without DNA extraction or sample processing. EDTA, Heparin anticoagulated whole blood, and blood collection cards (such as Whatman 903® and FTA® blood collection cards) can be directly used for PCR amplification identification, which greatly shortens the detection time. The 2× SuperEasyTM Mix provided by this kit has strong inhibitor tolerance. The maximum amount of human blood used as a PCR template can reach 45% of the PCR system, and that of mouse blood can reach 20%, which can sensitively amplify blood samples. Genomic and exogenous DNA fragments of interest.
2× SuperEasyTM Mix-EDTA contains our company's unique Foregene D-Taq DNA Polymerase, dNTPs, MgCl2, Taq Reaction Buffer, PCR reaction enhancer, optimizer and stabilizer.
2× SuperEasyTM Mix (UNG) replaces dTTP with dUTP on the basis of 2× SuperEasyTM Mix, and at the same time adds a UNG enzyme (Uracil-N-glycosylase) that can degrade the template containing dUTP. Before the PCR reaction, the PCR product containing uracil is degraded by UNG enzyme. UNG enzyme will not have any effect on the template that does not contain uracil, so as to ensure the specificity and accuracy of amplification and prevent the possibility of large-scale genetic testing. PCR product contamination problems arise.
D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions.
D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems. The amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.
According to the different anticoagulants used, the products are divided into two categories: EDTA anticoagulation whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-EDTA) and Heparin anticoagulation whole blood direct PCR kit series (BloodSuperDirectTM PCR Kit series). -Heparin); the corresponding kit can also be selected according to the experimental needs, see the selection of kit types for details.
Kit conponents
| Blood SuperDirectTM PCR Kit(EDTA)-UNG | ||||
|
Kit composition |
TP-0412T | TP-04121 | TP-04122 | TP-04123 |
| 50 T | 200 T | 500 T | 2000 T | |
| 2× SuperEasyTM Mix(UNG)-EDTA | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml×12 |
| 6× DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 |
| Manual | 1 | 1 | 1 | 1 |
Feature
- No time-consuming and expensive DNA purification, no pretreatment, and whole blood as a PCR template.
- The system has strong amplification ability and can sensitively detect genomic and exogenous target DNA fragments in blood.
- 2× SuperEasyTM Mix(UNG)-EDTA High blood tolerance: up to 45% in human blood and 20% in mouse blood.
- The sample is completely closed, and there is no need to worry about sample contamination and false positive PCR results. Anti-pollution PCR system 2×SuperEasyTM Mix(UNG)-EDTA can effectively eliminate the pollution caused by PCR products and ensure the specificity and accuracy of amplification.
Application
Specifically designed for direct PCR identification of EDTA anticoagulated whole blood. (Including: human blood, rat blood, chicken blood, bird blood, cow blood, dog blood, etc.).
Kit Component information
- 2× SuperEasyTM Mix(UNG)-EDTA: On the basis of 2× SuperEasyTM Mix-EDTA, dUTP is used to replace dTTP, and at the same time, UNG enzyme that can degrade the template containing dUTP is added, which can effectively prevent the contamination of PCR amplification products. Contains D-Taq DNA Polymerase specially modified by Fuji Bio, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer, etc. For PCR reactions, simply add the appropriate anticoagulated whole blood, primers, and ddH2O to the corresponding 2× SuperEasyTM Mix(UNG)-EDTA for PCR reactions.
- 6× DNA Loading Buffer: This Loading Buffer does not contain SDS. It is recommended to use the 6× DNA Loading Buffer provided with the kit when performing agarose gel electrophoresis in order to obtain good electrophoresis results. Do not use Loading Buffer containing SDS, otherwise there will be a large group of tail lights in the lane during electrophoresis, which will affect the experimental results.
Work Flow
Transportation
The whole transportation process with low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃.
Storage
This kit should be stored at -20°C.
- 2× SuperEasyTM Mix(UNG)-EDTA, if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).
- 6× DNA Loading Buffer,It can be stored at 4°C or -20°C for a long time.
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