Lab Use I II-UNG Direct PCR Kits One Step Plant Seed Direct PCR Plus Kit
Lab use Plant Seed Direct PCR Plus Kit I II-UNG Direct PCR kits one step
Product Pictures
Specifications:
50T,200T,500T,2000T
Product components:
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Plant Seed Direct PCR Plus Kit II Polysaccharide polyphenol plant seed (large and medium-sized) direct PCR kit |
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| Kit composition | TP-0317T | TP-03171 | TP-03172 | TP-03173 | |
| 50 T | 200 T | 500 T | 2000 T | ||
|
Part I |
Buffer SSP1 | 24ml | 96ml | 120ml×2 | 480ml×2 |
| Buffer SP2 | 3ml | 10ml | 25ml | 100ml | |
| 6× DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 | |
|
Part II |
Buffer SSP2 | 8ml | 32ml | 80ml | 80ml×4 |
| 2× Seed PCR EasyTM Mix | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml×12 | |
| Manual | 1 | 1 | 1 | 1 | |
Features&advantages:
| Easy | - No time-consuming and expensive DNA purification is required. |
| Low requirement | - The sample demand is small, just take a single seed. |
| Simple | - No special treatments such as grinding and crushing are required, and the operation is simple. |
| Specific | - The optimized PCR system enables PCR to have higher specificity and stronger tolerance to PCR reaction inhibitors. |
Product Descprition
This product uses a unique lysis reaction system, which can
- quickly release genomic DNA from plant seed samples with high polysaccharides and polyphenols (such as bananas, cotton, etc.) for use in PCR reactions, so it is particularly suitable for large-scale genetic testing.
- In order to meet the needs of different detection tests, this kit can use multiple types of samples (such as whole seeds, small tissue cuts, or ground samples of multiple seeds) for experiments.
- Among them, for the seed samples that still need to be germinated, you can choose to cut out micro-tissue pieces (1-5mg) other than the seed embryos for experiments; for experiments that require sampling and testing in a large number of samples, you can choose to mix multiple samples and combine them.
- After grinding into particles with a diameter of about 0.5mm, the experiment can be carried out (at least 0.1% of the target sample can be detected).
Work Flow:
Limitation
- Amplified fragment is ≤1kb；If it exceeds 1kb, the amplification efficiency will decrease or the amplification will fail.
If the PCR products is used for sequencing, it is recommended that the PCR products be purified first.
The PCR product may have some mutations, if it is used for gene cloning, please be aware.
A tail is randomly added to the 3'end of the PCR product.
Storage and Shelf life:
Transportation:
The whole process of low-temperature ice box transportation, to ensure that the kit Part II is in a state of <4 ℃.
Storage:
Part I of this kit should be stored at room temperature or 2-8°C.
Part II should be stored at -20°C.
Product Descprition
This product uses a unique lysis reaction system, which can quickly release genomic DNA from plant seed samples with high polysaccharides and polyphenols (such as bananas, cotton, etc.) for use in PCR reactions, so it is particularly suitable for large-scale genetic testing. In order to meet the needs of different detection tests, this kit can use multiple types of samples (such as whole seeds, small tissue cuts, or ground samples of multiple seeds) for experiments. Among them, for the seed samples that still need to be germinated, you can choose to cut out micro-tissue pieces (1-5mg) other than the seed embryos for experiments; for experiments that require sampling and testing in a large number of samples, you can choose to mix multiple samples and combine them. After grinding into particles with a diameter of about 0.5mm, the experiment can be carried out (at least 0.1% of the target sample can be detected).
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