Plant Leaf Direct PCR Plus Kit

High Effective Sensitive Plant Leaf Plus Direct PCR Kits For Polysaccharide polyphenol plants

Specifications:

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Features&advantages:

- No time- consuming and expensive DNA purification

- Less material

- Simple— the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding

- 2× Mix— reducing sample loading errors and reaction system preparation time

- Fast— template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes

- High throughput— lysis reaction can be completed in 96-well PCR plate

Description:

This product uses a unique lysis buffer system to lyse plant leaves. The lysate can be used as a template without purification. After adding the primers, the PCR Mix of this kit can be used for amplification.

Kit components:

Kit parameters:

Application: Identification of transgenic plants, genotyping, etc.

Sample: plant leaves rich in polysaccharide or polyphenol

Sample size: Diameter 2-3mm (direct method), diameter 5-7mm (lysis method)

Detection range: PCR product ≤1kb

Work Flow:

Electropherogram:

Storage and Shelf life:

Part I of this kit should be stored at room temperature or 2-8℃.

vReagents Buffer PS1, Buffer P2, 6×DNA Loading Buffer can be stored for 12 months under dry conditions; if needed to be stored for a longer time, the reagents can be stored at 2-8℃.

Part II of this kit should be stored at -20℃.

vReagents Buffer PS2, 2×Leaf PCR EasyTM Mix, if used frequently, can also be stored at 4℃ for short-term storage (use up within 10 days).