High Sensitivity MPCC RT QPCR Machine Thermal Cycler Reaserch Lab Use
Thermal Cycler Mini Real-Time system SF4 qPCR Machine Reaserch lab Use
Descripation:
As an important technology in scientific research and molecular diagnosis, real-time fluorescent quantitative PCR is widely used to meet the needs of rapid and timely detection and small-throughput scientific research and teaching. Foregene launched the ForeQuant SF series, an ultra-fast portable fluorescent quantitative PCR instrument system, which uses innovative circulating liquid refrigeration technology combined with Peltier temperature control technology. The operating software is simple, the body is light and compact, and it can quickly and accurately complete quantification without site restrictions. PCR-related applications.
Specifications:
| Temperature control | Optical inspection | ||
| Sample capacity | 2x8 wells(0.2ml single tube,8 strip tube) | Excitation light source | Monochromatic high-efficiency LED |
| Reaction volume | 10-50 ul | Detection Systems | high sensitivity MPCC |
| Thermal cycle technology | Peltier | Detection mode | ultra-fast hole-by-hole scanning |
| Cooling mode | Liquid circulation refrigeration | Fluorescent channels | tandard two channels(FAM/VIC), optional four channels(FAM/VIC/ROX/CY5) |
| Maximum heating and cooling rate | 8 ℃ per second | Detection time | ＜ 30 mins (combined with FOREGENE fast kit) |
| Temperature accuracy | ±0.2℃ | Sensitivity | With single-copy gene, can distinguish 1.33-fold copy number difference in single-plex reaction |
| Temperature uniformity | ±0.2℃@60℃±0.2℃@90℃ | Dynamic Range | 10 orders of magnitude |
Parameters:
| Hardware and other parameters | |||
| Noise | ＜50dB | Size | 205x190x98mm(LxWxH) |
| Power supply | 220V/optional 12 volt 10A mobile power supply | Software systems | Windows |
| Software functions | |||
| Amplification curves, absolute quantitative, qualitative analysis, dissolved curves | |||
Reaction Block:
ForeQuant SF is designed with 16 well position, which can be compatible with the common 0.2ml quantitative PCR transparent reaction tube and the transparent 8-strip reaction tube. The temperature uniformity between wells can be guaranteed (± 0.2 ℃) by cooperating with the auxiliary heating plate.
Signal acquisition system:
ForeQuant SF employs a new patented integrated optical signal scanning and detection system based on Fresnel lenses, high-efficiency MPPC and maintainable high-efficiency LEDs.
In an ordinary convex lens, the corners become dark and blurred when transmitted light. This is because the refraction of light only occurs at the interface of the medium. The convex lens plate is relatively thick, and the linear propagation of light in the medium will cause light attenuation. The Fresnel lens removes the linear part of the ordinary convex lens and only retains the curved surface of refraction.
A large amount of material can be saved while achieving the same concentration effect. A Fresnel lens looks like a piece of glass with an infinite number of concentric lines (called Fresnel zones), but it can achieve the effect of a convex lens. If the projected sources are parallel, then it can converge to preserve the brightness of the entire image.
MPPC can measure very weak radiant power at nanoscale wavelengths in low-level photometry and spectroscopy. It combines high gain, low noise, high frequency response and large signal receiving area. It is a photosensitive vacuum device with extremely high sensitivity and ultra-fast time response.
Packing list:
Please check the items in the package one by one according to the type and quantity in the packing list.
Storage and transportation environment:
Temperature: -20℃ ~ 55℃,the instrument packaging in the transport box；
Humidity: ≤85% RH
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