Description:
This kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality total RNA from cells cultured in 96, 24, 12, and 6-well plates.
The kit provides an efficient DNA-Cleaning Column, which can easily separate the supernatant and cell lysate, bind and remove genomic DNA. The operation is simple and time-saving.
The RNA-only Column can efficiently bind RNA with a unique formula. A large number of samples can be processed simultaneously.

 
 
Specifications:
50 Preps, 200 Preps

 
Kit components:

 
 
Features&advantages:
- The whole process is operated at room temperature (15-25℃), without ice bath and low temperature centrifugation.
- The whole kit is RNase-Free, no need to worry about RNA degradation.
- DNA-Cleaning Column specifically binds DNA, so that the kit can remove genomic DNA contamination without adding additional DNase.
- High RNA yield: RNA-only Column and unique formula can efficiently purify RNA.
- Fast speed: easy to operate and can be completed in 11 minutes.
- Safety: No organic reagent is required.
- High quality: The purified RNA is of high purity, free of protein and other impurities, and can meet various subsequent experiments.
 
Kit application:
It is suitable for extraction and purification of total RNA from cultured cells in 96, 24, 12, and 6-well plates.

 
Work Flow:

 
Diagram:

The agarose gel battery diagram of Cell Total RNA Isolation Kit treated the above different numbers of cells,20μl volume elution, take 2μl purified total RNA 1%.

 
Storage and Shelf life:
The kit can be stored for 12 months at room temperature (15–25 ℃) or 2–8 ℃ for longer time(24 months).
Buffer cRL1 can be stored at 4 ℃ for 1 month after adding 2-hydroxy-1-ethanethiol(optional).
 
Notice:
- All procedures are carried out at room temperature (15-25℃) , including centrifugation. Do not use ice bath or centrufuge at low-temperature (4℃) .
- The sample should avoid repeated freezing and thawing, otherwise it will lead to the degradation of the extracted RNA and the yield of RNA will also decrease.
- The yield and quality of RNA is tightly related to sample size and volume of elution. For every 500μL of buffer cRL1, the recommended maximum cell volume is 10⁶.
- Before using the kit, please add 2-hydroxy-1-ethanethiol to Buffer cRL1 (10μL 2-hydroxy-1-ethanethiol per 1mL Buffer cRL1). If the extracted RNA is not used to clone full-length cDNA, but only used for other downstream operations such as qPCR or sequencing analysis, it is not necessary to add 2-hydroxy-1-ethanethiol, and the result will not be affected.
- Before using the kit, please add anhydrous ethanol to buffer cRL2 and Buffer RW2 referring to the label on the reagent bottle for the dosage.
- The volume of elution should be no less than 20μL, otherwise it will influence RNA yield.
- Please check if there is any precipitate in the Buffer cRL1 and Buffer RW1. If the precipitate can be seen after storage at low temperature, the solution should be placed at room temperature(15-25℃) or 37℃ for period of time. Use the solution after dissolving and blending.