No Need RNA Extraction Unique Lysis Buffer System Part I/II Plant Seed Direct PCR Kit

Description:

This product uses a unique lysis buffer system to lyse plant seeds. The lysate can be used as a template without purification. After adding the primers, the PCR Mix of this kit can be used for amplification.

Specifications:

50×20µl rxns,200×20µl rxns,500×20µl rxns,2000×20µl rxns

Kit components:

Features&advantages:

-No time-consuming and expensive DNA purification(Time, labor and cost saved!)

-Less material

-Simple—the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding

-2 X Mix—reducing sample loading errors and reaction system preparation time

-Fast—template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes

-High throughput—lysis reaction can be completed in 96-well PCR plate

Kit parameters:

Application: Identification of transgenic plants, genotyping, etc.

Sample: Plant seeds or tissues with low polysaccharide and polyphenol content.

Sample size: Single whole seed, 1-100mg plant tissue

Detection range: PCR product ≤1kb

Work Flow:

Electropherogram:

Storage and Shelf life:

-Part I of this kit should be stored at room temperature or 2-8℃.

vReagents Buffer SP1, Buffer SP2, 6× DNA Loading Buffer, under dry conditions, can be stored for 12 months. If you need to store the kit for a longer time, you can store it at 2-8℃.

-Part II of this kit should be stored at -20℃.

vReagent 2× Seed PCR Easy Mix, if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).

APPENDIX I

The comparison between Direct PCR and traditional PCR: