Quick and efficient RT-qPCR Easy^TM (One Step)-Taqman qPCR Kit for lab use

Product Descprition

Foregene One-Step RT-PCR Easy series products realize the integrated reaction from RNA to double-stranded DNA, that is, reverse transcription and PCR amplification are completed in the same reaction centrifuge tube and the same reaction system, thus the experimental steps simplified, the experimental plan optimized, and the experimental efficiency improved.

RT-qPCR Easy^TM (One Step)-Taqman using Foregene HotStar Taq DNA Polymerase, the optimized Taqman qPCR system can quickly and specifically perform Real Time RT-qPCR quantitative detection of trace RNA templates.

Product components:

Features&advantages:

• One-step kit enables reverse transcription and PCR to be carried out in the same tube, only need to add template RNA, specific PCR primers and RNase-Free ddH₂O.
•  Real-time quantitative analysis of viral RNA or trace RNA can be carried out quickly and accurately.
• The kit uses a unique Foregene reverse transcription reagent and Foregene HotStar Taq DNA Polymerase combined with a unique reaction system to effectively improve the amplification efficiency and specificity of the reaction.
• The optimized reaction system makes the reaction have higher detection sensitivity, stronger thermal stability, and better tolerance.
• RT-qPCR Easy^TM (One Step)-Taqman kit comes with ROX internal reference dye, which can be used to eliminate signal background and signal errors between wells, which is convenient for end users to use in different models of quantitative PCR instruments

Kit component information

• 2× RT-qPCR Easy^TM Mix-Taqman: Foregene Reverse Transcriptase, RNase Inhibitor, Foregene HotStar Taq DNA Polymerase, optimized dNTPs, Mg²⁺, stabilizer, enhancer, and optimizer.
• ROX Reference Dye: Generally used on Real Time PCR thermal cyclers of companies such as ABI, Stratagene, etc., to adjust the difference among PCR tubes caused by PCR sample loading errors. The concentration of ROX Reference Dye required by different instruments is different, and the user can add it according to the recommended concentration of the instrument.

Product quality control:

In accordance with FOREGENE’s Total Quality Management System (FOREGENE’s Total Quality Management System), each batch of RT-qPCR Easy (One Step)-Taqman qPCR kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.

Form 1:RT-qPCR system preparation

Storage and Shelf life:

The kit should be stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.