Quick and easy one-step Cell Direct RT qPCR Kit SYBR GREEN I and Taqman​

Product Descprition

This kit uses a unique lysis buffer system that can quickly release RNA from cultured cell samples for RT-qPCR reactions, thereby eliminating the time-consuming and laborious RNA purification process. The RNA template can be obtained in just 7 minutes. The 5×Direct RT Mix and 2×Direct qPCR Mix-SYBR reagents provided by the kit can quickly and effectively obtain real-time quantitative PCR results.

5×Direct RT Mix and 2×Direct qPCR Mix-SYBR have strong inhibitor tolerance, and the lysate of the samples can be used as the template for RT-qPCR directly. This kit contains the unique RNA high-affinity Foregene reverse transcriptase, and Hot D-Taq DNA polymerase, dNTPs, MgCl₂, reaction buffer, PCR optimizer and stabilizer.

Product components:

Features&advantages:

• Simple and effective : with Cell Direct RT technology, RNA samples can be obtained in just 7 minutes.
• The sample demand is small, as low as 10 cells can be tested.
• High throughput: it can quickly detect RNA in cells cultured in 384, 96, 24, 12, 6-well plates.
• DNA Eraser can quickly remove released genomes, greatly reduce the impact on subsequent experimental results.
• Optimized RT and qPCR system makes the two-step RT-PCR reverse transcription more efficient and PCR more specific, and more resistant to RT-qPCR reaction inhibitors.

Kit parameters:

Application:

-Scope of application: cultured cells.

-RNA released by sample lysis: only applicable to the RT-qPCR template of this kit.

-The kit can be used for the following purposes: gene expression analysis, verification of siRNA-mediated gene silencing effect, drug screening, etc.

Product quality control:

In accordance with FOREGENE’s Total Quality Management System (FOREGENE’s Total Quality Management System), each batch of Cell Direct RT-qPCR kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.

Storage and Shelf life:

Part I of this kit should be stored at 4℃; Part II should be stored at -20℃.

Foregene Protease Plus II should be stored at 4℃,do not freeze at -20℃.

Reagent 2×Direct qPCR Mix-SYBR should be stored at -20℃ in the dark; if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).