Fast RT Easy II Master Premix Reverse Transcriptase kit for cDNA synthesis

Product Descprition

The RT Easy^TM II Reverse Transcriptase kit is a universal cDNA first-strand synthesis kit designed and developed based on reliable experiments, suitable for most sources of RNA.

• Simple and effective
• Reliable performance
• Cost-effective

Product components:

Features&advantages:

• Efficient reverse transcription system, it only takes 20 minutes to complete the synthesis of the first strand cDNA.
• High-sensitivity reverse transcription system, pg-level templates can also get high-quality cDNA.
• The reverse transcription system has high thermal stability, the reaction temperature of the system can be as high as 50 ℃, and it has good reverse transcription performance.
• Even impure RNA samples (alcohol up to 60%, guanidine salt up to 750mM) can be subjected to reverse transcription reaction.
• 2× RT OR-Easy^TM Mix has added optimized reverse transcription primers (Random Primer, Oligo(dT)₁₈ Primer).

Kit Application:

• Used directly in Real Time PCR to quantitatively analyze gene expression
• It can quickly and accurately analyze small amounts of RNA such as RNA viruses
• Reverse transcription of RNA templates with high GC content or complex secondary structure

Product quality control:

In accordance with FOREGENE’s Total Quality Management System (FOREGENE’s Total Quality Management System), each batch of RT Easy^TM II kits is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.

Precautions: (Please read the precautions carefully before using the kit)

• For templates, it is recommended to use RNA extracted from fresh samples or stored at -80℃ (RNA should avoid repeated freezing and thawing).
• In order to avoid RNase contamination, the experiment operation should be carried out in the RNase-Free space; the pipette tips and PCR centrifuge tubes used must be RNase-Free; and disposable gloves and masks should be worn.
• Before use, put 2× RT OR-Easy^TM Mix on ice to completely melt, flick and mix well before use; the preparation of the system should be operated on an ice bath to improve the performance of the kit and the specificity of PCR amplification performance.
• RT Easy^TM II has already added reverse transcription primers with optimized ratios, so there is no need to add any additional primers.

Storage and Shelf life:

1. Transportation conditions

The whole process of low-temperature ice box transportation, to ensure that the kit is in a state of <4°C.

2. Storage conditions

RT Easy^TM II is stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.