This COVID 19 RT PCR Test kit uses Real-time RT PCR technology (rRT-PCR) for qualitative detection of SARS-CoV-2 nucleic acids in human nasopharyngeal or oropharyngeal swab samples.

This primer and probe sets are designed for the detection of the conserved sequences of SARS-CoV-2 (ORF1ab gene, N gene and E gene). The kit contains an Internal Control that is used to assess specimen quality. Real-Time PCR technology utilizes polymerase chain reaction (PCR) for the amplification of specific target sequences and target specific probes for the detection of the amplified RNA. The probes are labelled with fluorescent reporter and quencher dyes.

◮Specifications: 48 Rxns/kit, 96 Rxns/kit

◮ Specifificity:96.72%

Sensitivity:97.92%

◮Applicable specimen types

Nasopharyngeal Swab or Oropharyngeal Swab

◮ Applicable instrument

ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S

◮ Direct PCR

No need for nucleic acid extaction kit and System, completed 96 specimen in 1 hour or so.

◮ Low equipment requirement and highly flflexible use

Only need Real-Time PCR system.

◮ LoD and high sensitivity

Detection sensitivity can reach the lowest level as 500 copies/ml.

Method 1: Direct PCR method

Method 2: Viral RNA Isolation + PCR

Method 1: Direct PCR method using Sample Release Agent

Detection procedure:

Sealed from light and stored at -20±5℃;

Shelf life:

1 year