Spin Column Third Generation DNA Isolation Plant With High Purity And Yield
Spin Column Third Generation DNA Isolation Plant DNA Isolation Kit With High Purity And Yield
Description:
This kit uses a DNA-only column that can specifically bind DNA, Foregene protease and a unique buffer system, which greatly simplifies the purification of plant genomic DNA. High-quality genomic DNA can be can be obtained within 30 minutes, which avoid the degradation of genomic DNA.
The DNA-only silica gel membrane used in the spin column is Foregene's unique new material, which can effectively and specifically bind to DNA, and maximize the removal of RNA, impurity proteins, ions, polysaccharides, polyphenols and other organic compounds.
Specifications:
50 Preps, 100 Preps,250 Preps
Product components:
| Plant DNA Isolation Kit | |||
| Kit component | DE-06111 | DE-06112 | DE-06113 |
| 50次 | 100次 | 250次 | |
| Buffer PL1 | 35ml | 70ml | 180ml |
| Buffer PL2* | 35ml | 70ml | 180ml |
| Buffer PW* | 30ml | 60ml | 150ml |
| Buffer WB | 25ml | 50ml | 125ml |
| Buffer EB | 10ml | 20ml | 50ml |
| Foregene Protease | 1.25ml | 2.5ml | 6.5ml |
| DNA-Only Column | 50 | 100 | 250 |
| Manual | 1 | 1 | 1 |
*: Buffer PL2, Buffer PW contains irritating base, please pay attention to gloves and carry out protective measures when operating.
Product Information
| Format | Spin column | Purification component | Foregene column, reagent |
| Flux | 1-24 samples | Time per prep | ~30 min (24 samples) |
| Centrifuge | Desk centrifuge | Plant lysate separation | Centrifugal separation |
| RNA Purification column loading | 80μg | Liquid content of spin column | 800μl |
| Elution volume | 100-200 μL | Plant sample processing volume | 100 mg |
Features&advantages:
-No RNase contamination: The DNA-Only Column provided in the kit makes it possible to remove RNA from genomic DNA without additional RNase during the experiment, thereby preventing the laboratory from being contaminated by exogenous RNase.
-Fast speed: Foregene Protease has higher activity than similar proteases and digests tissue samples faster;
-Simple: the genomic DNA purification operation can be completed in 30 minutes.
-Convenient: The centrifugation is performed at room temperature, 4℃ centrifugation and ethanol precipitation of DNA is not required.
-Safety: no organic reagent is used.
-High quality: The purified genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
Kit application:
It is suitable for extraction and purification of genomic DNA from fresh or frozen plant tissues.
Kits component information
- BUFFER PL1: Provides plant tissue cracking environments.
- Foregene Protease: Protein in the enzymatic plants in the environment of Buffer PL1.
- BUFFER PL2: Factory ForeGene Protease and provides a DNA upper column environment.
- BUFFER PW: Remove protein, RNA and other impurities in DNA.
- BUFFER WB: Remove residual salt ions in DNA.
- BUFFER EB: Elution of DNA on the purified column membrane.
- Genetological DNA in specific adsorption lysis.
Work Flow:
Diagram:
Storage and Shelf life:
The kit can be stored for 12 months at room temperature (15-25℃), or 2-8℃ for longer time.
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