Laboratory Research Animal Tissue DNA Isolation Kit Purified Spin Column
Laboratory Research Use Animal Tissue DNA Isolation Kit purified DNA spin column DNA purification
Product Descprition
Animal Tissue DNA Isolation kit uses a DNA-only column that can specifically bind DNA, Foregene protease and a unique buffer system. High-quality genomic DNA can be extracted from various cultured cells and animal tissues within 30 to 50 minutes.
The DNA-only silica gel membrane used in the spin column is Foregene's unique new material, which can effectively and specifically bind to DNA, and maximize the removal of RNA, impurity proteins, ions and other organic compounds in cells. 5-80μg high-quality genomic DNA can be purified from 10-50mg tissue.
Specifications:
250 Preps
Product components:
| Animal Tissue DNA Isolation Kit | |||
| Kit content | DE-05011 | DE-05012 | DE-05013 |
| 50T | 100T | 250T | |
| Buffer L1 | 20ml | 40ml | 100ml |
| Buffer L2* | 20ml | 40ml | 100ml |
| Buffer PW* | 25ml | 50ml | 125ml |
| Buffer WB | 25ml | 50ml | 125ml |
| Buffer EB | 10ml | 20ml | 50ml |
| Foregene Protease | 1.25ml | 2.5ml | 6.5ml |
| DNA-Only Column | 50 | 100 | 250 |
| Manual | 1 | 1 | 1 |
*: Buffer L2 and Buffer PW contain irritating desalted salt. Please wear gloves and take relevant protective measures when operating.
Product information
| Model | Spin-column type | Purification components | Foregene Spin column, reagent |
| Flux | 1-24 samples | Preparation time |
30-50min (24 samples) |
| Centrifuge | Table centrifuge | Tissue hydrolysate separation | centrifugal separation |
| Purification column DNA load carrying capacity | 80μg | Liquid volume of spin column | 800μl |
| Elution volume | 100-200μl | Bacterial liquid processing capacity | 10-50mg |
Features&advantages:
-No RNase contamination: The DNA-Only Column provided in the kit makes it possible to remove RNA from genomic DNA without additional RNase during the experiment, thereby preventing the laboratory from being contaminated by exogenous RNase.
-Fast speed: Foregene Protease has higher activity than similar proteases and digests tissue samples faster;
-Simple: the genomic DNA purification operation can be completed in 50 minutes.
-Convenient: The centrifugation is performed at room temperature, 4℃ centrifugation and ethanol precipitation of DNA is not required.
-Safety: no organic reagent is used.
-High quality: The purified genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
Kit application:
Animal Tissue DNA Isolation kit is suitable for the extraction and purification of genomic DNA from a variety of fresh or frozen cultured cells and animal tissues.
Work Flow:
Diagram:
Precautions
- The sample should avoid repeated freezing and thawing, otherwise the extracted DNA fragments will be smaller and the extraction volume will also decrease.
- Before use, carefully check whether there is precipitation in Buffer L1, Buffer L2 and Buffer PW. If there is precipitation, please dissolve it at 37°C and mix well before use.
- Before using the kit, be sure to check whether Buffer WB has been added with absolute ethanol according to the instructions. Add 60 mL of absolute ethanol (DE-050111), 120 mL of absolute ethanol (DE-05012), and 300 mL of absolute ethanol (DE-05013) to Buffer WB before use.
- Elution volume: Buffer EB should not be less than 100μL, otherwise it will affect the DNA yield.
- All experimental steps are carried out at room temperature (15-25℃).
Storage and Shelf life:
The kit can be stored for 12 months at room temperature (15-25℃), or 2-8℃ for longer time.
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