General Plasmid Mini DNA Isolation Kit Purification Column Plasmid DNA Extraction
General Plasmid Mini DNA Isolation Kit DNA Isolation Kits Purification Column Plasmid DNA Extraction
Description:
Foregene's General plasmid mini Kit adopts DNA-only purification column technology and efficient SDS lysis method to obtain high-quality Plasmid DNA from bacteria Based on the company's unique DNA-only purification column and unique formula reagent, can maximize the removal of RNA, without adding RNA enzyme, can remove RNA effect, so that the laboratory from RNA enzyme pollution but also can efficiently remove impurities in protein ions and other organic compounds in bacteria The actual plasmid DNA yield and purity were related to the host bacteria species and culture conditions bacterial lysis and plasmid copy number (high or low copy) and other factors.
General plasmid mini Kit is suitable for plasmid DNA extraction of Gram-negative bacteria. The obtained plasmid DNA has high purity, no RNase and very low ion content, which can meet the requirements of various conventional experiments, such as invertase PCR library construction and sequencing (ddH2O eluting plasmid DNA is recommended).
Specifications:
50Preps, 100 Preps, 250 Preps
Kit components:
| General Plasmid Mini Kit | |||
| Kit content | DE-01001 | DE-01002 | DE-01003 |
| 50T | 100T | 250T | |
| Buffer S1 | 10ml | 20ml | 50ml |
| Buffer S2 | 10ml | 20ml | 50ml |
| Buffer S3 | 25ml | 50ml | 125ml |
| Buffer PW | 25ml | 50ml | 125ml |
| Buffer WB2 | 15ml | 30ml | 75ml |
| Buffer EB | 10ml | 20ml | 50ml |
| DNA-Only Column | 50 | 100 | 250 |
| Manual | 1 | 1 | 1 |
| Model | Spin-column type | Purification components | Foregene Spin column, reagent |
| Flux | 1-24 samples | Preparation time |
20min |
| Centrifuge | Table centrifuge | Tissue hydrolysate separation | centrifugal separation |
| Purification column DNA load carrying capacity | 60μg | Liquid volume of spin column | 800μl |
| Elution volume | 50-200μl | Bacterial liquid processing capacity | 1-5ml |
Features&advantages:
| No RNase added | Remove RNA without adding RNase |
| Convenient | the centrifugation is performed at room temperature, 4℃ centrifugation and ethanol precipitation of DNA is not required. |
| Fast | the operation can be completed in 20 minutes |
| Safe | no organic reagent used |
| High purity | OD260/280≈1.7-1.9 |
Kit parameters:
| Downstream applications | transformation, restriction enzyme digestion, sequencing, PCR, transfection and passage of cells, etc. |
| Sample | overnight culture broth |
| Sample size | 1-15ml of bacterial liquid |
| Maximum DNA binding capacity of purification column | 80 μg |
| Elution volume | 50-100 μl |
Work Flow:
Diagram:
Precautions
- The amount of bacterial solution should not be too much. OD600=2.0-3.0 is recommended, and the amount should not exceed 5ml, otherwise the yield and purity of extracted plasmid DNA will be affected.
- Before use, carefully check whether there is precipitation in Buffer S2 and Buffer S3. If there is precipitation, please dissolve it in 37℃ and mixed before use.
- Before using the kit, be sure to check whether anhydrous ethanol has been added to Buffer WB2.
- Elution volume: should not be less than 50μl, otherwise it will affect plasmid DNA yield.
- All the experimental steps were carried out at room temperature (15-25℃), including centrifugation steps by bench centrifuge at room temperature.
Storage and Shelf life:
This kit can be stored for 12 months under dry conditions at room temperature (15-25℃). If it needs to be stored for a longer time, it can be stored in 2-8℃.
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