Lnc-RT Hero^TM I(With gDNase) first strand cdna synthesis reverse transcription system

Description:

Lnc-RT Hero^TM I (With gDNase) is a reverse transcription system specially developed for lncRNA to quickly remove genomic DNA contamination. 5×gDNase Mix can quickly remove the remaining genome in RNA at 42°C for 2 minutes, effectively avoiding the interference of genome on qPCR results.

5×L-RT HeroTM Mix contains Foregene LncRNA Reverse Transcriptase specially developed by Foregene, which is a new type of reverse transcriptase specifically for lncRNA and other long RNA complex templates, with stronger RNA affinity and higher reversal recording efficiency. The optimized system makes the reverse transcription rate faster and can easily transcribe RNA templates with high GC content and complex secondary structure. The first strand cDNA synthesis can be completed at 42°C in 15 minutes.

Specifications:

25×20μl Rxns, 50×20μl Rxns

Kit components

Kits component information

-5×gDNase Mix: gDNA removing agent, it is necessary to use the reagent in accordance with the operating instructions before the RT reaction is performed (the internal glycerol content may not be frozen, and it may not be frozen, which belongs to normal phenomena).

- 5×L-RT HEROTM MIX: Contains Foregene Lncrna Reverse Transcriptase, RNase Inhibitor, DNTPS, Stabilifer, Enhancer, Optimizer, Oligo (DT) 18 primer).

Features&advantages:

-Efficient ability to remove gDNA, which can remove gDNA in the template within 2 minutes.

-It can efficiently reverse transcription of lncRNA. When the reverse transcription product is subjected to qPCR, the Ct value is lower than that of conventional reverse transcription system.

-Efficient reverse transcription system, it only takes 15 minutes to complete the synthesis of the first strand cDNA.

-Complex templates: templates with high GC content and complex secondary structure can also be reversed with high efficiency.

-High-sensitivity reverse transcription system, pg-level templates can also get high-quality cDNA.

-The reverse transcription system has high thermal stability, the optimal reaction temperature is 42℃, and it still has good reverse transcription performance at 50℃.

Template RNA requirements

It is best to use RNA extracted from fresh samples or preserved at -80 °C.

- Template integrity: good integrity, no degradation.

- Template purity: IONS, proteins, EDTA, ethanol, phenols and other journals contained in RNA will affect reverse transcriptase activity and ultimately affect reverse transcription effects.

Template RNA dosage

- For lncRNA: 10ng-1 μg total RNA/20 μl system.

- For general RNA: 0.1pg-1 μg total RNA or 0.01pg-0.1 μg mRNA/20 μl system.

Workflow:

Storage and Shelf life:

The Super Premix PCR Kits should be stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.