Safe Fast Molecular Biology Reagent FFPE DNA Isolation Kits For Genomic DNA Purification
Safe and fast molecular biology reagent FFPE DNA Isolation Kits for genomic DNA purification
Description
This kit is dedicated to extract and purify high-quality genomic DNA from formaldehyde-fixed tissue or paraffin-embedded tissue.
The genomic DNA of the paraffin-embedded sample will be degraded due to the storage time or the environment, making it difficult to extract the genomic DNA. This kit adopts a unique optimized formula, which is suitable for extracting genomic DNA from paraffin-embedded samples with a high extraction success rate. The whole process does not need to use toxic phenol, chloroform or isopropanol precipitation.
This kit uses a spin column that can specifically bind DNA, a brand-new Foregene Protease Plus, and a unique buffer system, which can extract high-quality paraffin-embedded tissue genomic DNA within 2-5 hours.
Kit conponents
| FFPE DNA Isolation Kit | |||
| Kit contents | DE-05411 | DE-05412 | DE-05413 |
| 50 T | 100 T | 250 T | |
| Buffer FL1 | 20ml | 40ml | 100ml |
| Buffer FL2* | 20ml | 40ml | 100ml |
| Buffer PW* | 30ml | 60ml | 150ml |
| Buffer WB | 25ml | 50ml | 125ml |
| Buffer EB | 12.5ml | 25ml | 65ml |
| Foregene Protease Plus | 1.25ml x2 | 5ml | 12.5ml |
| DNA-Only Column | 50 sets | 100 sets | 250 sets |
| IFU | 1 piece | 1 piece | 1 piece |
*: Buffer FL2 and Buffer PW contain irritating chaotropic salts. Please wear gloves and take relevant protective measures during operation.
Features&advantages
- No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.
- Fast speed: Foregene Protease Plus has higher activity than similar proteases, and it digests tissue samples faster; the operation is simple, and the genomic DNA extraction operation can be completed within 2-5 hours.
- Convenience: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.
- Safety: no organic reagent extraction is required.
- High quality: The extracted genomic DNA fragments are large, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
Genomic DNA Application
The genomic DNA purified by the FFPE DNA Isolation Kit has high purity and can be used for routine molecular biology operations, such as: restriction enzyme digestion, PCR, Southern hybridization, library construction and other experiments.
Quality Control
According to FOREGEN's Total Quality Management System , each batch of paraffin-embedded tissue genomic DNA extraction kit is strictly tested for multiple times to ensure the reliability and stability of the quality of each batch of kits.
Product INFO
| Model | Spin-column type | Purification components | Foregene Spin column, reagent |
| Flux | 1-24 samples | Preparation time | 2-5h(24 samples) |
| Centrifuge | Table centrifuge | Tissue hydrolysate separation | centrifugal separation |
| Liquid volume of spin column | 800μl | Purification column DNA load carrying capacity | 30μg |
| Elution volume | 50-100μl | Tissue sample processing volume | 10-50mg |
Genomic DNA extraction yield and purity
The extraction rate of genomic DNA from paraffin-embedded tissue is related to the source of the tissue, storage conditions, storage time, dosage and other factors. The purity of the obtained genomic DNA meets the routine molecular biology experiment operations, and OD260/280 is 1.7-1.9.
Genomic DNA fragment size
The paraffin-embedded tissue genomic DNA extraction kit uses a silica membrane column to separate and purify genomic DNA from a variety of sample sources. The purified genomic DNA fragments will be distributed in a large range according to the storage method and time, and become diffuse bands. See the example below (for reference only).
Storage and Shelf life
1. This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.
Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to put the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.
2. Foregene Protease Plus solution has a unique formula that is active when stored at room temperature for a long time (3 months); it is more active and stable when stored at 4°C, so it is recommended to store it at 4°C, remember not to keep it at -20°C.
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