PCR Kits Premix System Containing Taq Enzyme High Efficiency
PCR kits premix system containing Taq enzyme high efficiency
Description:
This kit includes Foregene's unique new generation of Taq—Foregene Taq Hero, optimized dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The 2× PCR HeroTM Mix (dye) system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.
Specifications:
5ml, 10ml, 40ml
Kit(2× PCR HeroTM Mix) components:
|
0.1U Taq Hero/μl |
|
400μM dNTPs |
|
20mM Tris-HCl(pH8.8) |
|
100mM KCl |
|
5mM MgCl2 |
|
Other stabilizers and enhancers |
|
DNA Loading Buffer |
Features&advantages:
| Higher fidelity | 6 times that of ordinary Taq enzyme; |
| Faster amplification speed | the amplification speed is 5-10sec/kb, which is 6-12 times that of ordinary Taq enzyme; |
| More template adaptability | it can efficiently amplify various complex DNA templates with high GC content and difficult to amplify; |
| Higher Amplification efficiency | the number of amplification cycles is lower than that of ordinary Taq enzyme; |
| Stronger Environmental tolerance | placed at 37°C for a week, maintaining more than 90% activity; |
| Activity | It has 5’→3’ DNA polymerase activity and 5’→3’ exonuclease activity, without 3’→5’ exonuclease activity. |
Practical examples
1.Reaction system
| 2× PCR HeroTM Mix(dye) | 10μl |
| Template DNA | xμl |
| Primer 1(10μM) | 0.4μl |
| Primer 2(10μM) | 0.4μl |
| ddH2O | Fill to 20μl |
| Total volume | 20µl |
2.Reaction conditions
| Temperature | Time | Cycles |
| 94°C | 3min | 1 |
| 94°C | 10sec |
25-40 |
| 55-65°C | 10sec | |
| 72°C | ≥10sec (5-20sec/kb)1* | |
| 72°C | 5min | 1 |
1*: If using plasmid as template, the extension speed is 5sec/kb, we recommend using 10sec/kb; if using genomic DNA as the template, the extension speed is 10sec/kb, we recommend using 20sec/kb. If you use a highly complex template that is difficult to amplify, it is recommended to increase the extension time by 10sec/kb on the original basis.
Note: For 10µl and 20µl systems, if the PCR machine does not have a thermal cover, add an equal volume of mineral oil. The PCR reaction conditions are based on the specific conditions of the template, primer set, and the GC content and length of the primers to design the best reaction conditions, including annealing temperature, extension time, etc.
Kit application:
PCR amplification of DNA fragments, DNA labeling, DNA sequencing,PCR plus A tail
Storage and Shelf life:
It can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.
Instructions
PCR HeroTM(With Dye) is convenient and quick to use, and can avoid the pollution during the PCR operation and the experimental error caused by multiple preparations of the reaction system to the greatest extent. Use only half the volume of the reaction system (for example, if the reaction system is 20μl, take 10μl 2× PCR HeroTM Mix(dye)) of 2× PCR HeroTM Mix(dye), add template and primers, and add deionized water to make up Volume, make the PCR Mix concentration in the reaction system 1×, then the reaction can be carried out.
Practical examples
1.Reaction system
| 2× PCR HeroTM Mix(dye) | 10μl |
| Template DNA | xμl |
| Primer 1(10μM) | 0.4μl |
| Primer 2(10μM) | 0.4μl |
| ddH2O | Fill to 20μl |
| Total volume | 20µl |
2.Reaction conditions
| Temperature | Time | Cycles |
| 94°C | 3min | 1 |
| 94°C | 10sec |
25-40 |
| 55-65°C | 10sec | |
| 72°C | ≥10sec (5-20sec/kb)1* | |
| 72°C | 5min | 1 |
1*: If using plasmid as template, the extension speed is 5sec/kb, we recommend using 10sec/kb; if using genomic DNA as the template, the extension speed is 10sec/kb, we recommend using 20sec/kb. If you use a highly complex template that is difficult to amplify, it is recommended to increase the extension time by 10sec/kb on the original basis.
Note: For 10µl and 20µl systems, if the PCR machine does not have a thermal cover, add an equal volume of mineral oil. The PCR reaction conditions are based on the specific conditions of the template, primer set, and the GC content and length of the primers to design the best reaction conditions, including annealing temperature, extension time, etc.
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