High DNA extraction yield Mouse Tail SuperDirect PCR Kits for mouse direct PCR perform room temperature lysis
High DNA extraction yield Mouse Tail SuperDirect PCR Kits for mouse direct PCR perform room temperature lysis
Product Descprition
This kit uses a unique lysis buffer system to quickly release genomic DNA from mouse tail and mouse ear samples for PCR reaction, so it is particularly suitable for large-scale genetic testing.
The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at room temperature (20-25°C). No heating is required, and other processes such as protein and RNA removal are not required. The released trace amounts of DNA can be used as Template for PCR reaction.
2×M-PCR EasyTM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.
D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.
Product INFO:
| Model | Spin-column type | Purification components | Foregene Spin column, reagent |
| Flux | 1-24 samples | Preparation time |
60-90min (24 samples) |
| Centrifuge | Table centrifuge | Tissue enzymolysis isolation | centrifugal isolation |
| Purification column DNA load carrying capacity | 80μg | Liquid volume of spin column | 800μl |
| Elution volume | 100-200μl |
Mouse tail sample processing volume |
1-5ml |
Kit contents:
| Mouse Tail SuperDirectTM PCR Kit | |||||
| Kit contents | TP-0131T | TP-01311 | TP-01312 | TP-01313 | |
| 50T | 200T | 500T | 2000T | ||
| Part I | Buffer NP | 3ml | 10ml | 25ml | 100ml |
| Foregene Protease Plus I | 55μl | 220μl | 550μl | 1.1ml×2 | |
| 6× DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 | |
| Part II | 2× M-PCR EasyTM Mix | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml×12 |
| IFU | 1piece | 1piece | 1piece | 1piece | |
Features&advantages:
-No time-consuming and expensive DNA purification is required.
-The sample demand is small, just take mouse tails (or mouse ears with a diameter of 2-5mm) above 2mm.
-Easy to operate, it can be lysed without heating to inactivate the protease, and the lysed product can be directly subjected to PCR reaction.
-Foregene Mouse Tail Genomic DNA Extraction Kit (DE-05211) can be used to extract genomic DNA from the processed rat tail.
-Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.
Genomic DNA fragment size:
Mouse Tail DNA Mini Kit uses a silica membrane column to isolate and purify mouse tail genomic DNA. The purified genomic DNA fragments size are all around 23 kb. As shown in the figure:
Storage and Shelf life:
Part I of this kit should be stored at 2-8℃.
The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.
Reagent Foregene Protease Plus I has a unique formula. To ensure its better activity and stability, please store it at 4℃.
Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.
Part II of this kit should be stored at -20℃ .
Reagent 2×M-PCR EasyTM Mix, if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).
Work Flow:
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