Blood SuperDirectTM PCR Kit(UNG)-Heparin DNA Polymerase pcr kit pcr products
Blood SuperDirectTM PCR Kit(UNG)-Heparin DNA Polymerase pcr kit pcr products
Description
Blood SuperDirectTM PCR Kit(UNG)-Heparin uses a unique PCR buffer system, without DNA extraction or sample processing, it can perform direct PCR with a whole blood sample as a template. EDTA, Heparin anticoagulated whole blood and blood collection cards (such as Whatman 903® and FTA® blood collection cards) can be directly used for PCR amplification and identification, which greatly shortens the detection time. The 2× SuperEasyTM Mix provided by this kit has strong inhibitor tolerance. The maximum amount of human blood used as a PCR template can be up to 45% of the PCR system and 20% of mouse blood, which can sensitively amplify blood samples. Genomic and foreign target DNA fragments.
2× SuperEasyTM Mix-Heparin Contains our company's unique Foregene D-Taq DNA Polymerase, dNTPs, MgCl2, Taq Reaction Buffer, PCR reaction enhancer, optimizer and stabilizer.
2× SuperEasyTM Mix(UNG)-Heparin On the basis of 2×SuperEasyTM Mix-Heparin, dUTP was used instead of dTTP, and UNG enzyme (Uracil-N-glycosylase) that could degrade the dUTP template was added at the same time. Before the PCR reaction, the UNG enzyme is used to degrade the PCR product containing uracil. The UNG enzyme will not have any effect on the template that does not contain uracil, thereby ensuring the specificity and accuracy of amplification and preventing the possibility of large-scale genetic testing. Contamination of PCR products occurred.
D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.
According to the different anticoagulants used, this product is divided into two categories: EDTA anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-EDTA) and Heparin anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-Heparin); You can also choose the corresponding kit according to the needs of the experiment, see the selection of kit type for details.
Kit conponents
| Blood SuperDirectTM PCR Kit(UNG)-Heparin Heparin Anticoagulated Whole Blood Direct PCR Kit-UNG | ||||
| Kit Composition | TP-0422T | TP-04221 | TP-04222 | TP-04223 |
| 50 T | 200 T | 500 T | 2000 T | |
| 2× SuperEasyTM Mix(UNG)-Heparin | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml×12 |
| 6× DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 |
| Manual | 1 | 1 | 1 | 1 |
Feature
-The system has strong amplification ability and can sensitively detect the genome and foreign target DNA fragments in the blood.
- 2× SuperEasyTM Mix(UNG)-Heparin High blood tolerance: up to 45% in human blood and 20% in mouse blood.
-The sample is fully enclosed, so there is no need to worry about sample contamination and false positive PCR results. Anti-pollution PCR system 2×SuperEasyTM Mix (UNG), effectively eliminate the pollution caused by PCR products, and ensure the specificity and accuracy of amplification.
Application
Dedicated to direct PCR identification of Heparin anticoagulated whole blood. (Including: human blood, mouse blood, chicken blood, bird blood, cow blood, dog blood, etc.).
Transportation
The whole process of low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃.
Limitation
-The amplified fragment is less than or equal to 1kb; if it exceeds 1kb, the amplification efficiency will decrease or the amplification will fail.
-If the PCR product is used for sequencing, it is recommended that the PCR product be purified first.
-The PCR product may have some mutations, if it is used for gene cloning, please be aware.
- The PCR product may have some mutations, if it is used for gene cloning, please be aware.
-The PCR products obtained from the UNG anti-PCR product contamination system should not be used for gene cloning or sequencing.
- A tail is randomly added to the 3'end of the PCR product.
Storage
This kit is stored at -20°C.
The whole process of low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃.
-2× SuperEasyTM Mix(UNG)-Heparin,If used frequently, it can also be stored at 4°C for short-term storage (use up within 10 days).
-6× DNA Loading Buffer,It can be stored at 4°C or -20°C for a long time.
Work Flow
Kit composition information
- 2× SuperEasyTM Mix(UNG)-Heparin:On the basis of 2× SuperEasyTM Mix-Heparin, dUTP is used instead of dTTP, and the UNG enzyme that can degrade the dUTP template is added at the same time, which can effectively prevent the contamination of PCR amplification products. Contains the specially modified D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer, etc. In the PCR reaction, just add the appropriate anticoagulated whole blood, primers, and ddH2O to the corresponding 2× SuperEasyTM Mix(UNG)-Heparin to be used in the PCR reaction.
- 6× DNA Loading Buffer:The Loading Buffer does not contain SDS. It is recommended to use the 6× DNA Loading Buffer included with the kit when performing agarose gel electrophoresis in order to obtain good electrophoresis results. Do not use Loading Buffer containing SDS, otherwise there will be a large tail of bright light in the lane during electrophoresis, which will affect the experimental results.
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