Plant Leaf Direct PCR Plus Kit For plant leaves containing high polysaccharide and polyphenol components​

Description:

Plant Leaf Direct PCR Plus Kit adopts a unique lysis buffer system, which can rapidly release genomic DNA from leaf samples of plants with high polysaccharide and polyphenol content (such as cotton, banana, etc.) for PCR reaction. The leaves do not need to be ground or shredded when treated with lysis buffer, making them ideal for large-scale genetic testing.

The process of releasing genomic DNA from the lysis buffer can be completed within 5-10 minutes, without the need for other processes of deproteinization, RNA or secondary metabolites, and the released trace DNA can be used as a template for PCR reaction.

2× Leaf PCR EasyTM Mix has a strong resistance to PCR reaction inhibitors, and can directly use the cleavage product of plant material as a template for efficient and specific amplification. The reagent contains D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer, and stabilizer specially engineered for direct PCR reactions. It can be used in combination with direct lysate to detect samples quickly and easily, and has the characteristics of high sensitivity, strong specificity and good stability.

D-Taq DNA polymerase is a DNA polymerase specially developed for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems. The amplification speed can reach 2Kb/min. It is especially suitable for Direct PCR reaction.

According to the differences of plant samples, the products are divided into two categories: Plant Leaf Direct PCR Kit and Plant Leaf Direct PCR Plus Kit for polysaccharides and polyphenols; in the PCR detection stage, it can be According to the experimental needs, choose the common 2× Leaf PCR Easy^TM Mix or the 2× Leaf PCR Easy^TM Mix (UNG) which has the effect of preventing the contamination of PCR amplification products.

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Specifications:

50×20µl rxns,200×20µl rxns,500×20µl rxns,2000×20µl rxns

Kit components:

Features & advantages:

-No time-consuming and expensive DNA purification

-Less material

-Simple—the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding

-2X Mix—reducing sample loading errors and reaction system preparation time

-Fast—template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes

-High throughput—lysis reaction can be completed in 96-well PCR plate

Kit parameters:

Application: Identification of transgenic plants, genotyping, etc.

Sample: plant leaves

Sample size: Diameter 2-3mm (direct method), diameter 5-7mm (lysis method)

Detection range: PCR product ≤1kb

Kit Component Information

-Buffer PS1: Provides the environment required for plant leaf lysis reaction.

-Buffer PS2: Supplementation provides the environment required for the lysis reaction of polysaccharides and polyphenols in plant leaves.

Buffer P2: Neutralizes the lysate so that it does not interfere with subsequent PCR reactions.

-2× Leaf PCR EasyTM Mix: Contains Taq DNA Polymerase specially modified by Fuji Bio, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer, etc. For PCR reactions, simply add the appropriate lysis mix, primers, and ddH2O to the 2× Leaf PCR EasyTM Mix for PCR reactions.

-6× DNA Loading Buffer: This Loading Buffer does not contain SDS. It is recommended to use the 6× DNA Loading Buffer provided with the kit when performing agarose gel electrophoresis in order to obtain good electrophoresis results. Do not use Loading Buffer containing SDS, otherwise there will be a large tail of light in the swimming lane during electrophoresis, which will affect the experimental results.

Work Flow:

Electropherogram:

Storage and Shelf life:

Part I of this kit should be stored at room temperature or 2-8℃.

-Reagents Buffer P1, Buffer P2, 6×DNA Loading Buffer can be stored for 12 months under dry conditions; if needed to be stored for a longer time, the reagents can be stored at 2-8℃.

Part II of this kit should be stored at -20℃.

-Reagents Buffer 2×Leaf PCR Easy^TM Mix, if used frequently, can also be stored at 4℃ for short-term storage (use up within 10 days).