Foreasy Taq DNA Polymerase HotStar Taq for PCR/qPCR taq enzyme pcr kits for dna sequencing conventional pcr

Product Introduction

Foreasy Taq DNA Polymerase is a new Taq enzyme expressed in Escherichia coli engineering bacteria by gene recombination technology. The enzyme itself has a certain hot-start activity and can be used for conventional PCR and qPCR; it has 5'→3' DNA polymerase activity and 5'→3' exonuclease activity, but no 3'→5' exonuclease activity.

Component

Storage

-20 ± 5 °C for 2 years or at -80 °C for long-term storage.

Features

High specificity: The enzyme has a certain hot-start activity.

Fast Amplification: 10 sec/kb .

Highly adaptable template: can be used to efficiently amplify GC High value, various difficult-to-amplify DNA template.

Strong fidelity: ordinary Taq Enzyme 6 times.

Strong thermal stability: It can be placed at 37 °C for a week and maintains more than 90% activity

Application

Various PCR/qPCR systems and direct PCR systems

PCR amplification of DNA fragments

DNA labeling

DNA sequencing

PCR A-tailed

U Definition

1U: The amount of enzyme required to incorporate 10 nmol of deoxynucleotides into acid-insoluble matter using activated salmon sperm DNA as template/primer for 30 minutes at 74°C.

Activity Assay Conditions

1× Taq Reaction Buffer,1.5 mM MgCl₂；0.2 mg/mL activated calf thymus DNA,0.2 mM dNTPs.

Storage

20 mM Tris-HCl (pH 8.0),1 mM DTT,0.1 mM EDTA,50% glycerol,stabilizer.

2× Taq Reaction Buffer

Contains optimized ratios of Tris, KCl, MgCl2 and other ingredients.