Foreasy HS Taq DNA Polymerase Super HotStar Taq Enzyme Mix For PCR/QPCR
Foreasy HS Taq DNA Polymerase Super HotStar Taq Enzyme Mix for PCR/qPCR
Product Description
Foreasy HS Taq DNA Polymerase is a new Taq enzyme expressed in Escherichia coli engineering bacteria by gene recombination technology . After the enzyme is treated with a special process, it's activity is inhibited before thermal activation, thereby inhibiting the non-specific amplification caused by the non-specific annealing of primers or primer dimers under low temperature conditions. This product is suitable for highly specific PCR Reaction, M ultiple x PCR , high GC content ( > 60% ) ,with secondary structure or other strong background genomics amplification and large-scale genomics amplification detection. The enzyme has 5' → 3' DNA polymerase activity and 5' → 3' exonuclease activity, but no 3' → 5' exonuclease activity.
product composition
| component | IM-01021 | IM-01022 | IM-01023 |
| Foreasy HS Taq DNA Polymerase (5 U/μL) |
5000 U (1 mL) |
50 KU (10 mL) |
500 KU (100 mL) |
| 2× Taq Reaction Buffer |
25mL ×5 |
250 mL ×5 |
500 mL × 25 |
Storage
-20 ± 5 °C for 2 years or at -80 °C for long-term storage.
Features
v High specificity: The enzyme with high hot-start activity.
v Fast Amplification: 10 sec/kb .
v High template adaptability : can be used to efficiently amplify High GC value and various difficult-to-amplify DNA template.
v Strong fidelity: The fidelity is 6 times of ordinary Taq Enzyme .
Product Usage
v Various PCR/qPCR System and direct PCR system
v PCR Amplified DNA Fragment
v DNA mark
v DNA Sequencing
v PCR plus A tail
Activity Definition
1U : The amount of enzyme required to incorporate 10 nmol of DNA into acid-insoluble matter using activated salmon sperm DNA as template / primer, 74 °C, 30 minutes .
Activity Assay Conditions
1× Taq Reaction Buffer , 1.5 mM MgCl 2 ; 0.2 mg/mL activated calf thymus DNA , 0.2 mM dNTPs .
Storage conditions
20 mM Tris-HCl (pH 8.0) , 1 mM DTT , 0.1 mM EDTA , 50% glycerol , stabilizing agent.
2× Taq Reaction Buffer
Contains optimized proportions of Tris , KCl , MgCl 2 and other ingredients.
Example of use:
reaction system
| System additions | added dose |
| Foreasy HS Taq DNA Polymerase | 0.4 μL _ |
| 2 × Taq Reaction Buffer | 25 μL _ |
| Template DNA | X μL _ |
| dNTPs (10 mM each) | 1 μL _ |
| Primer-F | 1 μL _ |
| Primer-R | 1 μL _ |
| ddH 2 O | To 50 μL _ |
| Total Volume | 50 μL _ |
Reaction conditions
| Temperature | Reaction Time | Cycle time |
| 37°C | 5 min | 1 |
| 94°C | 5 min | 1 |
| 94°C | 10 Secs |
40 |
| 60°C | 10 Secs |
Note: For 10 µL and 20 µL systems, add an equal volume of mineral oil if the thermal cycler does not have a heat lid .
PCR reaction conditions vary depending on the structural conditions of templates, primers, and the like. In the specific operation, it is necessary to design the optimal reaction conditions, including annealing temperature, extension time, etc., according to the specific conditions such as the template type, the size of the target fragment, the base sequence of the amplified fragment, and the GC content and length of the primer.
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