Foreasy RNase Inhibitor

RNase Inhibitor for RNA protection

China IVD Factory Low Price High Production Foreasy RNase Inhibitor For RNA Protection

Product Introduction

Foreasy RNase Inhibitor is a new mouse-derived RNase inhibitor expressed in E. coli engineered bacteria using gene recombination technology. The inhibitor inhibits RNase activity by non-competitively combining with RNase 1:1, thereby protecting RNA integrity , and can effectively inhibit the RNase A, B, C activity , but not RNase T1, T2, H, etc.; It is reversible of the binding of RNase Inhibitor and RNase , and the complex can be dissociated by urea and sulfhydryl reagents, so that RNase renatures and the inhibitor is irreversibly inactivated.

This product has the same application as the enzyme derived from human placenta. Foreasy RNase Inhibitor has high thermostability. It maintains high activity and inhibits RNase In the reverse transcription system using highly thermostable reverse transcriptase (such as: Foreasy Reverse Transcriptase)

Product Component

Type

Storage

Store at -20±5°C for 2 years or at -80°C for long-term storage.

Application

Foreasy RNase Inhibitor can be used in all experiments with RNase contamination or interference to protect RNA from degradation; in experiments, Foreasy RNase Inhibitor will not interfere or affect the experiment itself. For instance:

Synthesis of the first strand of cDNA, RT-PCR (direct RT-qPCR and direct RT-PCR)

uIn vitro transcription/translation

Inhibition of RNase activity during RNA isolation and purification

RNase protection experiment

U Definition

1 U : The amount of enzyme required to inhibit 50% of the activity of 5 ng RNase A is defined as 1 activity unit (U). (The inhibitory activity is determined by inhibiting the ability of RNase A to hydrolyze Cyclic2’,3’-CMP)

Precautions

vForeasy RNase Inhibitor activity has a wide range of pH adaptation (both pH5-9 are active), and the activity is best at pH7-8.

vForeasy RNase Inhibitor is inactivated at 65°C and above.

vForeasy RNase Inhibitor requires at least 1 mM DTT to be active. The DTT content of this product is 8 mM.

Recommended dosage

Final concentration of reaction system: 0.5-1 U/μL