Foregene Co., Ltd.
                                                                                                           
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Since 2010
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Molecular Biology Lab Reagent General Plasmid Mini Kit Large DNA Isolation Kit

Price Negotiable
Price: Negotiable
MOQ: 1 kit
Delivery Time: 5-8 days
Brand: FOREGNE
Product Description

 

 

Molecular biology lab reagent General Plasmid Mini Kit large DNA isolation kit

 

Description:
 
The FOREGENE’S nucleic acid extraction and purification series products can be extracted and purified to high-quality nucleic acids from a variety of sources and complex component samples. The DNA extraction series products are based on DNA-Only silica membrane purification columns and unique formula reagents that can purify high-quality DNA from a variety of samples.
The General Plasmid Mini Kit uses DNA-Only purification column technology and an efficient SDS lysis formula to obtain high-quality plasmid DNA from bacteria within 20 minutes. The DNA-Only silica gel membrane used in the spin column is a unique new material of FOREGENE, which can adsorb DNA efficiently and specifically,and remove RNA, impurity proteins, ions and other organic compounds in cells to the greatest extent. The maximum binding capacity of plasmid DNA of DNA-Only purification column is 60μg DNA. The amount of plasmid DNA actually extracted is related to the volume of the bacterial solution, the number of plasmid copies (high copy, low copy), strains, culture conditions and other factors.

 
Kit components:

 

General Plasmid Mini Kit
Kit content DE-01001 DE-01002 DE-01003
50T 100T 250T
Buffer S1 10ml 20ml 50ml
Buffer S2 10ml 20ml 50ml
Buffer S3 25ml 50ml 125ml
Buffer PW 25ml 50ml 125ml
Buffer WB2 15ml 30ml 75ml
Buffer EB 10ml 20ml 50ml
DNA-Only Column 50 100 250
Manual 1 1 1
 

 
Features&advantages:

-No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove the RNA in the plasmid DNA without adding RNase during the experiment, avoiding the contamination of the laboratory by exogenous RNase.
-Fast speed: Plasmid DNA extraction can be completed within 20 minutes.
-Safe: No organic reagent extraction is required.
-High quality: The plasmid obtained by extraction has no RNA, no RNase and extremely low ion content, which can meet the requirements of various experiments.

 

Product Quality Control:

 

In accordance with FOREGENE's Total Quality Management System, each batch of General plasmid mini kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.

 

Kit components information:

 

- Buffer S1:Provides bacterial lysis environment.

- Buffer S2:Lyse bacteria, denature protein and DNA.

- Buffer S3:Precipitates protein and genomic DNA, and provides a column environment for plasmid DNA.

- Buffer PW:: Remove impurities such as protein and RNA from plasmid DNA.

- Buffer WB2:Remove residual salt ions from plasmid DNA.

- Buffer EB:Elute the plasmid DNA on the purification column membrane.

- DNA-Only Column:Specific adsorption of plasmid DNA in the lysate.

 

Product information:

 

Model Spin-column type Purification components Foregene Spin column, reagent
Flux 1-24 samples Preparation time

20min

(24 samples)

Centrifuge Table centrifuge Tissue hydrolysate separation centrifugal separation
Purification column DNA load carrying capacity 60μg Liquid volume of spin column 800μl
Elution volume 50-200μl Bacterial liquid processing capacity 1-5ml


Storage and Shelf life:
 
This kit can be stored for 12 months under dry conditions at room temperature (15-25℃). If it needs to be stored for a longer time, it can be stored in 2-8℃.
 

Get in Touch

Have questions about our products or want to discuss a custom order? Our team is ready to help you.

Company Foregene Co., Ltd.
Location Building D2, block A, Tri-medical innovation center, No.18, North Bayi Road, Yongning Town, Wenjiang District, Chengdu City, Sichuan Province, P.R.China
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