Blood Genomic DNA Mini Kit DNA Isolation Kits Clinical Testing Reagents
Blood Genomic DNA Mini Kit quality DNA Isolation Kits Clinical testing reagents
Description:
The blood sample contains a large number of red blood cells and platelet cells, which makes blood processing more troublesome, and the purity and quantity of blood genomic DNA purified by conventional methods is low. The kit uses Foregene Protease Plus and unique BL1 and BL2 buffer systems, so anticoagulated blood and coagulated blood samples can be completely digested in a short time, thereby minimizing DNA degradation and obtaining the maximum amount of genomic DNA. The fast blood processing system and simple spin column operation greatly simplify the purification of blood genome, and high-quality and high-purity genomic DNA can be obtained within 40 minutes.
The DNA-only silica gel membrane used in the spin column of the kit is Foregene's unique new material, which can efficiently and specifically bind DNA, and can remove RNA, impurity proteins, ions and other organic compounds in cells to the greatest extent. The maximum capacity of the spin column is 80 μg DNA. The obtained DNA can be used in molecular biology experiments, such as restriction digestion, PCR, Southern hybridization and DNA library construction.
Kit conponents:
| Blood DNA Mini Kit | |||
| Kit component | DE-05111 | DE-05112 | DE-05113 |
| 50T | 100T | 250T | |
| Buffer BL1 | 15ml | 30ml | 75ml |
| Buffer BL2 | 15ml | 30ml | 75ml |
| Buffer DC | 100ml | 200ml | 500ml |
| Buffer PW | 25ml | 50ml | 125ml |
| Buffer WB1 | 15ml | 30ml | 75ml |
| Buffer EB | 10ml | 20ml | 50ml |
| Foregene Protease Plus | 1.25ml | 2.5ml | 6.5ml |
| DNA-Only Column | 50 | 100 | 250 |
| Manual | 1 | 1 | 1 |
*: Buffer BL2 and Buffer PW contain irritating liquid salt. Please wear gloves and take relevant protective measures when operating.
Product information:
| Model | Spin-column type | Purification components | Foregene spin column,reagent |
| Throughput | 1-24 samples | Preparation time | 40-50min(24 samples) |
| Centrifuge | Table centrifuge | Sample enzymatic hydrolysate separation | Centrifugal separation |
| Purification column DNA load carrying capacity | 800μl | Purification column DNA loading capacity | 80μg |
| Elution volume | 100-200μl | Blood sample throughput | 50-1000μl |
Features&advantages:
- No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.
- Fast speed: Foregene Protease Plus has higher activity than similar proteases, and digests tissue samples faster; the operation is simple, and the genomic DNA extraction operation can be completed within 40 minutes.
- Convenience: The centrifugation is performed at room temperature, 4°C centrifugation or ethanol precipitation of DNA is not required.
- Safety: No organic reagent extraction is used.
- High quality: The extracted genomic DNA fragments are large, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
Kit application:
It is suitable for extraction and purification of genomic DNA from fresh or frozen anticoagulated whole blood.
Diagram:
Work Flow:
Storage and Shelf life:
The kit can be stored for 12 months at normal temperature (15-25 ℃) under dry conditions. If placed in 2-8 ℃, it can be stored a longer time. Foregene Protease Plus solution has a unique formula and has activity when stored at room temperature for a long time (3 months).Its activity and stability will be better if stored at 4℃, so it is recommended to store it at 4℃, remember not to store it at -20℃.
Note: the solution is easy to precipitate if stored at low temperature. The solution in the kit must be placed at room temperature for a period of time before use. If necessary, it can be preheated in a water bath for 10 minutes at 37℃ to dissolve the precipitation and be mixed well before use.
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