IGRA TB Elisa Test Kit Interferon Gamma Release Assay For Mycobacterium Tuberculosis
Interferon-gamma Release Assay for Mycobacterium tuberculosis,IGRA - TB Elisa kit
ELISA Components
| Microplate Strips | 12x 8 well |
| Human IFN-γ Standard, lyophilised (Blue lid) | 2 x vial |
| Diluent | 1 x 40mL |
| Biotin Labeled Antibody (Yellow cap) | 1 x 6mL |
| Enzyme Conjugate 100X Concentrate (Brown bottle) | 1 x 130μL |
| Wash Buffer 10X Concentrate | 1 x 40mL |
| Chromogen Solution A | 1 x 6mL |
| Chromogen Solution B | 1 x 6mL |
| Stopping Solution |
1 x 6mL |
In Vitro Diagnostic Use Only
This kit contains reagents sufficient for testing of maximum of 28 specimens in a test run.
1. INTENDED USE
This kit is an enzyme-linked immunosorbent assay for quantitative detection of Interferon Gamma (IFN-γ) that responds to in-vitro stimulation by Mycobacterium tuberculosis antigens in fresh human whole blood. It is intended for use as an aid in the diagnosis of tuberculosis (TB) infection.
2. PRINCIPLE OF THE TEST
This kit uses the principle of IGRA combined with enzyme linked immunosorbent assay (ELISA) to
measure specific antigen mediated immune response strength. The antigens selected for stimulation is
specific fragments that pathogenic Mycobacterium tuberculosis have, but BCG vaccine and other
Mycobacterium do not have. The patients infected with Mycobacterium tuberculosis have specific T
lymphocytes which can identify these antigens and T lymphocytes will be stimulated to proliferate and
release cytokines such as IFN-γ. This kit uses ELISA method for quantitative detection the specific IFN-γ
released by cells to determine Mycobacterium tuberculosis infection.
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