Hot Sale Syphilis Elisa Kit (TP Elisa Kit) 96 Tests/Kit
Catalog No.: BE801A
For the detection of Total Antibody to Treponema Pallidum in human serum or plasma (in vitro) .

1. INTENDED USE

The Anti-TP ELISA is a qualitative enzyme immunoassay for the in vitro detection of Treponema Pallidum (TP) infection, based on the detection of total antibody to Treponema Pallidum in human serum or plasma. It is intended for screening of blood donors and diagnosing patients related to infection with TP.

2.COMPONENTS

Materials provided with the kits:

3.ASSAY PROCEDURE 

1. Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer (20×)with 19 volumes of distilled water, mix well.
2. Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negative control, 2 wells as positive control. After dispensing 50μL of Sample Diluent , dispense 50μL of sample or negative control or positive control to the respective wells. Gently vibrating the plate.
3. Incubate: Cover the Microplate with plate cover and incubate the Coated Microplate in a thermostat-controlled water-bath or microplate incubator at 37℃ for 60 minutes.
4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper.
5. Add Conjugate: Add 100μL of conjugate to each well (except for the blank well).
6. Incubate: Cover the Microplate and incubate the plate at 37℃ for 30 minutes.
7. Wash the Plate: Repeat the wash procedure as in step 4.
8. Add Substrate: Add 50μL of Substrate Solution A and 50μL of Substrate Solution B to each well, mix well. Cover and incubate at 37℃ for 30 minutes.
9. Stop reaction: Add 50μL Stop Solution to each well, mix well.
10. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.