Human HBeAg Elisa Kit 96 Test/Kit

1. HBeAg ELISA Kit
Catalog No.: BE103A

Enzyme-linked immunosorbent assay for the detection HBeAg in serum or plasma

2. SUMMARY AND PRINCIPLE OF THE TEST

1. The Hepatitis B e antigen is found to be related to Hepatitis B viral infection. HBeAg is usually found in the early phase of Hepatitis B viral 2. infection.

2. The titer of HBeAg rises rapidly during the period of virus replication. The presence of HBeAg correlates with increasing numbers of infectious viruses (Dane particles) and the presence of viral specific DNA polymerase in serum.

3. During the HBeAg positive stage, hepatitis B patients are at increasing risk of transmitting the virus to their contacts.

4. Persistent presence of HBeAg in the hepatitis B virus carrier is often associated with chronic active hepatitis.

3. REAGENTS

Materials provided with the kits:
1.Microtiter Well coated with monoclonal anti-HBe: 96 tests in one bag.
2.Negative Control: 0.5ml HBeAg Negative Control.
3.Positive Control: 0.5ml HBeAg Positive Control.
4.Enzyme Conjugate:.6 ml containing HRP-conjugated-anti-HBe
5.Wash Buffer Concentrate (20 x): 20 ml for 96 tests. The buffer should be diluted 20 times with distilled water before use.
6.Substrate Solution A: 6 ml HRP Substrate for 96 tests.
7.Substrate Solution B: 6 ml TMB Chromagen Substrate for 96 tests.
8.Stop Solution: One bottle of 6 ml 2N Sulfuric Acid.