Human HBsAb ELISA Kit Enzyme Immunoassay Test

HBsAb ELISA Kit
Catalog No.:BE102A

Enzyme-linked immunosorbent assay for the detection of antibody to Hepatitis B Virus surface antigen in serum or plasma.

1. SUMMARY

Hepatitis B is an infectious illness caused by hepatitis B virus (HBV) which infects the liver of hominoidea, including humans, and causes an inflammation called hepatitis. HBV is excreted in body fluids such as semen, saliva, blood and urine in persons with acute or chronic infection. The hepatitis B virus is known as a blood-borne virus because it is transmitted from one person to another via blood or fluids contaminated with blood. Classification of a hepatitis B infection requires the identification of several serological markers expressed during three phases (incubation, acute and convalescent) of the infection.

ASSAY PROCEDURE:

It is strongly advised to analyze each specimen and controls in duplicate. All the reagents should equilibrate to room temperature before use.

• For each test, set one blank well as background control, two positive and two negative controls.
• Add 50μl positive control, Negative control, and Specimen into their respective wells. Neither samples nor HRP-Conjugate should be added into the Blank well.
• Add 50 μl Enzyme Conjugant to each well except the Blank. Mix it gently by swirling the microtiter plate on flat bench for 1 minute. Do not add Enzyme Conjugate to the blank well.
• Cover wells with seal paper, Place the microtiter plates into a humidified box, and incubate at 37°C for 30 minutes.
• Wash each well 5 times by filling each well with diluted wash buffer, then inverting the plate vigorously to get all water out and blocking the rim of wells on absorbent paper for a few seconds.
• Add one drop (50 ul) of Substrate Solution A (HRP-substrate) to each well, then add one drop (50 ul) of Substrate Solution B (TMB) to each well. Mix gently and incubate at 37°C for 15 minutes. .
• Add 1 drop (50 ul) of Stop Solution to each well to stop the color reaction. Read the OD value at 450 nm/630 nm with dual filter plate reader. It is option to read the OD value at 450 nm with single filter plate reader. (using the OD value of the blank well to correct all the OD reading from all wells)

3. REAGENTS

Materials provided with the kits: