RUO Serum Amyloid A (SAA) Assay（Latex Immunoturbidimetric）
Research test for the quantitative determination of SAA Kit in human serum on automate chemistry analyzers
SAA (Serum Amyloid A) is is an acute-phase protein. During
inflammation, infectious and non-infectious diseases, its concentration in the blood can rise sharply within a few hours, and can rise to 1000 times the initial concentration. SAA is
related to HDL, and it can It regulates the metabolism of HDL
during inflammation. A particularly important feature of SAA is
that its degradation products can be deposited in different organs
in the form of amyloid A fibrils. This is a serious complication in
chronic inflammatory diseases; Elevated serum amyloid A is also
seen in atherosclerosis, diabetic nephropathy, acute myocardial
infarction, coronary heart disease, and chronic kidney disease
Components
| Contents | components | Concentration |
|
R1 |
PBS Buffer NaCl Surfactant Antiseptic |
0.1 mol/L Titer Titer Titer |
|
R2 |
Latex particles coated with SAA antibody PB Buffer NaCl Surfactant Antiseptic |
>0.5mg/L
0.1 mol/L Titer Titer Titer |
|
Calibrator /Control |
SAA Bovine serum albumin NaCl Surfactant Antiseptic |
See Value Sheet Titer Titer Titer Titer |
【Procedure】
Reagent preparation:
R1: Ready for use; R2: Ready for use. Wavelength:570nm Temperature:37℃
Direction: Increase
| Blank Tube | Calibration Tube | Sample Tube | |
| Sample | － | － | 2ul |
| Calibrator | － | 2ul | － |
| Deionized water | 2ul | － | － |
| R1 | 200μl | 200μl | 200μl |
| Mix, incubate at 37℃ for 5 minutes. | |||
| R2 | 40μl | 40μl | 40μl |
|
Mix, incubate at 37℃ for 5 minutes, zero setting for blank tube, read the absorbance △A calibrator and △A sample |
|||
Calibration
It is recommended to use the SAA Calibrators for calibration. Dissolve the lyophilized SAA calibrator with 1ml distilled water, and dilute to 6 calibrators as follows,
| Dilute | 1 | 2 | 3 | 4 | 5 | 6 |
| CAL (μl) | 200 | 100 | 50 | 50 | 25 | 0 |
|
Normal Saline (μl) |
0 | 100 | 150 | 350 | 375 | 200 |
| ratio | 1 | 1/2 | 1/4 | 1/8 | 1/16 | 0 |
Calibration Method:Spline or other non-linear method
Quality control
It is recommended to use the SAA Quality Control level 1 and level 2 for daily quality control.
Two levels of controls should be assayed at least once a day. Values obtained should fall within a specified range.
Each laboratory should establish its own internal Quality Control scheme and procedures for corrective action if controls do not recover within the acceptable tolerances.
Calculation:
The analyzer automatically calculate the analyte concentration of each sample.
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