For Laboratory Or Hospital High Accuracy Ferritin Elisa Test Kit

INTENDED USE

  The Ferritin assay is an enzyme-linked immunosorbent assay (ELISA for the in vitro quantitative determination of ferritin in human serum. This assay is useful in diagnosis and screening of anemia. For professional use only.

Test principle

Sandwich principle. Total duration of assay: 80 minutes.

• Sample, Anti-Ferritin coated microwells and enzyme labeled Anti-Ferritin are combined.

• During the incubation, ferritin presents in the sample is allowed to react simultaneously with the two antibodies, resulting in the ferritin molecules being sandwiched between the solid phase and enzyme-linked antibodies.

• After washing, a complex is generated between the solid phase, the Ferritin within the sample and enzyme-linked antibodies by immunological reactions.

• Substrate solution is then added and catalyzed by this complex, resulting in a chromogenic reaction. The resulting chromogenic reaction is measured as absorbance.

• The absorbance is proportional to the amount of ferritin in the sample.

Materials provided

• Ferritin Coated Microplate - symbol with mouse anti-ferritin.

• Ferritin Calibrators - symbol FERR PLATE FERR CAL A-F 8 x 12 strips, 96 wells, pre-coated 6 vials, 1 mL each, ready to use; Concentrations: 0(A), 20(B), 50(C), 100(D), 300(E) and 600(F) ng/mL.

• Ferritin Enzyme Conjugate - symbol FERR CONJ peroxidase) labeled mouse monoclonal anti-ferritin.

• Substrate - symbol SUBSTRATE 1 vial, 11 mL of HRP (horseradish 1 vial, 11mL, ready to use, (tetramethylbenzidine) TMB. STOP • Stop Solution - symbol 1 vial, 6.0 mL of 1 mol/L sulfuric acid.

• Wash Solution Concentrate - symbol PBS-Tween wash solution.

• IFU - 1 copy.

• Plate Lid: 1 piece.