T4 Triiodothyronine ELISA TEST KIT

INTENDED USE

Immunoassay for the in vitro quantitative determination of thyroxine in human serum.

Materials provided

• Coated Microplate, 8 x 12 strips, 96 wells, pre-coated with T4 derivant.

• Calibrators, 6 vials, 1 ml each, ready to use; Concentrations: 0(A), 2.5(B), 5(C), 10(D), 15(E) and 30(F) μg/dL.

• Enzyme Conjugate, 1 vial, 6.0 ml of HRP (horseradish peroxidase) labeled mouse monoclonal T4 in Tris-NaCl buffer containing BSA (bovine serum albumin). Contains 0.2% ProClin300 preservative. ANS 1.5 mg/mL.

• Substrate, 1 vial, 11ml, ready to use, (tetramethylbenzidine) TMB.

• Stop Solution, 1 vial, 6.0 ml of 1 mol/l sulfuric acid.

• Wash Solution Concentrate, 1 vial, 25 ml (40X concentrated), PBS-Tween wash solution.

• IFU, 1 copy.

• Plate Lid: 1 piece.

Materials required (but not provided)

• Microplate reader with 450nm and 620nm wavelength absorbent capability.

• Microplate washer.

• Incubator.

• Plate shaker.

• Micropipettes and multichannel micropipettes delivering 50μl with a precision of better than 1.5%.

• Absorbent paper.

• Distilled water

Storage

• Store at 2-8℃.

• Place unused wells in the zip-lock aluminum foiled pouch and return to 2-8 °C, under which conditions the wells will remain stable for 2 months, or until the labeled expiry date, whichever is earlier.

• Seal and return unused calibrators to 2-8 °C, under which conditions the stability will be retained for 1 month, for longer use, store opened calibrators in aliquots and freeze at -20 °C. Avoid multiple freeze-thaw cycles.

• Seal and return all the other unused reagents to 2-8 °C, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.