Laboratory Or Hospital High Precision TB T cell ELISA TEST KIT
TB T cell ELISA TEST KIT
INTENDED USE
Diagnostic Kit for Mycobacterium Tuberculosis Specific T Cells (ELISpot) (QB-Spot) is used in vitro to detect effector T cells activated by Mycobacterium tuberculosis-specific antigen stimulation in lymphocytes separated from anti-coagulated human fresh peripheral vein blood.
QB-Spot uses the ELISpot method for the diagnosis of clinically suspected tuberculosis.
QB-Spot treat T cells separated the subject’s whole blood with Mycobacterium tuberculosis-specific antigen’s stimulation in vitro. T cells infected with Mycobacterium tuberculosis can produce a specific reaction to such stimulation in vitro, turning into specific effector T cells and producing IFN-γ. Mycobacterium tuberculosis specific effector T Cells can be tested by ELISpot method and counted by
| Product details | Description |
| Delivery | Within 48 hours |
| Packaging Specifications | 8 x 12 strips, 96 wells |
| Country Of Origin | China |
| Manufacturer | 18 months |
| Preservation method | 2℃-8℃ |
| Specimen | Whole blood |
| Assification | class1 |
| Type | Elisa Test Kit |
Major Components
Major components in the kit:
| Name | Specification | Quantity | Components |
| Pre-coated Culture Plate | 96 wells | 1 plate | Composed of 12 × 8-well strips, coated with anti-human IFN-γ monoclonal antibody |
| Mycobacterium Tuberculosis Specific Antigen | 4 mL/bottle | 1 bottle | The main component are early secreting antigen target 6 (ESAT-6), culture filtrate protein 10 (CFP-10) and peptide fragment antigen Rv3615c |
| Positive Stimulus | 4 mL/bottle | 1 bottle | The main component is phytohemagglutinin (PHA) |
| Biotinylated Antibody Concentrate (250×) | 50 µL/vial | 1 vial | Contain biotinylated anti-human IFN-γ monoclonal antibody |
| Enzyme Conjugate Concentrate (200×) | 60 µL/vial | 1 vial | Contain horseradish peroxidase-labeled streptavidin |
| Assay Solution | 25 mL/bottle | 1 bottle | The main component is a phosphate buffer solution (PBS) and bovine serum albumin(BSA) |
| Wash Solution Concentrate (20x) | 50 mL/bottle | 1 bottle | The main component is a phosphate buffer solution (PBS) |
| Chromogenic Substrate Concentrate (50x) | 250 µL/ vial | 1 vial | The main component is 3-amino-9-ethylcarbazole (AEC) |
| Chromogenic Substrate Dilution | 12 mL/bottle | 1 bottle | The main components are PBS and dimethylsulfoxide (DMSO) |
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