Inhibin A ELISA TEST KIT

INTENDED USE

The Inhibin A assay is an enzyme-linked immunosorbent assay (ELISA) for the in vitro quantitative determination of dimeric inhibin A in human serum. This assay can be used as an aid in the diagnosis and monitoring of various hormonal reproductive disorders. For professional use only.

SUMMARY

Inhibins are dimeric glycoproteins composed of an α-subunit and either a βA (inhibin A) or a βB-subunit (inhibin B). The inhibin α-subunit has N-linked glycosylation sites and the glycosylation is essential for inhibin bioactivity (FSH suppressing activity). Inhibins are mainly produced in gonads and provide negative regulation of FSH secretion, but also have paracrine/autocrine actions in gonads and adrenal gland. As they sharing the β-subunits with activins, it is now recognized that actions of inhibins are associated with their antagonism of activin signaling. In both females and males, inhibin inhibits FSH production. Inhibin does not inhibit the secretion of GnRH from the hypothalamus.1,2 However, the overall mechanism differs between the sexes: In females, inhibin is produced in the gonads, pituitary gland, placenta, corpus luteum and other organs. FSH stimulates the secretion of inhibin from the granulosa cells of the ovarian follicles in the ovaries. In turn, inhibin suppresses FSH. Inhibin B reaches a peak in the early- to mid-follicular phase, and a second peak at ovulation. Inhibin A reaches its peak in the mid-luteal phase. Inhibin secretion is diminished by GnRH, and enhanced by insulin-like growth factor-1 (IGF-1). In males, it is secreted from the Sertoli cells,3 located in the seminiferous tubules inside the testes. Androgens stimulate inhibin production; this protein may also help to locally regulate spermatogenesis.

In Vitro Diagnostic

Use Only

• Inhibin A Coated Microplate - symbol INHA PLATE 8 x 12 strips, 96 wells, pre coated with mouse monoclonal βA subunit antibody.

• Inhibin A Enzyme Conjugate - symbol INHA CONJ 1 vial, 11 mL of HRP (horseradish peroxidase) labeled mouse monoclonal anti-inhibin α subunit antibody in Tris-NaCl buffer containing BSA (bovine serum albumin). Contains 0.1% ProClin300 preservative.

• Inhibin A Calibrators - symbols INHA CAL A-F 6 vials, 1.5 mL each, ready to use, Concentrations: 0(A), 20(B), 50(C), 200(D), 600(E) and 1200(F) pg/mL.

• Inhibin A Sample Buffer A - symbol 0.2% ProClin 300 preservative

• Inhibin A Sample Buffer B - symbol INHA BUFFER A INHA BUFFER B 1 vial, 3.0 mL. Contains 1 vial, 3.0 mL. Contains Bovine serum albumin (BSA), animal serum (goat, mouse), and surfactant.

• Substrate - symbol SUBSTRATE (tetramethylbenzidine) TMB. STOP 1 vial, 11mL, ready to use,

• Stop Solution - symbol 1 vial, 6.0 mL of 1 mol/L sulfuric acid.

• Wash Solution Concentrate - symbol WASH 40X concentrated), PBS-Tween wash solution.

• IFU - 1 copy.

• Plate Lid: 2 pieces.