Product Description:

Triiodothyronine (T3) is a crucial thyroid hormone in serum that regulates metabolism. Its concentration is pivotal for diagnosing euthyroid, hyperthyroid, and hypothyroid conditions. Most T3 is bound to transport proteins like TBG, prealbumin, and albumin, but free T3 (FT3) is the biologically active form. Unlike total T3, FT3 levels are unaffected by changes in binding proteins, making its measurement independent of such variations. In normal thyroid function, FT3 levels remain steady even if total T3 fluctuates due to alterations in carrier proteins. This stability makes FT3 concentrations a more reliable indicator of clinical status. Age-related studies show a gradual decline in mean FT3 values from younger to older individuals.

Features:

• Product Name: FT3(Quantitative)
• ELISA Assay Kit for Medical Diagnosis
• Storage Temperature: 2-8°C
• Country of Origin: China
• Manufacturer: Biovantion
• Kit Size: 96 Tests
• Shelf Life: 18 Months
• ELISA Diagnostic Test Kit for Infectious Diseases

Applications:

The FT3 assay is an enzyme-linked immunosorbent assay (ELISA) for the in vitro quantitative determination of free triiodothyronine (FT3) in human serum. The assay is useful in the diagnosis and treatment of thyroid disorders. For professional use only.

Test principle:

Competition principle. Total duration of assay: 80 minutes
• Sample, T3 derivant coated microwells and enzyme labeled Anti-T3 are combined.
• During the incubation, T3 derivant coated on microwells and FT3 present in the sample compete for binding to the enzyme labeled antibodies.
• After washing, a complex is generated between the solid phase and enzyme-linked antibodies by immunological reactions.
• Substrate solution is then added and catalyzed by this complex, resulting in a chromogenic reaction. The resulting chromogenic reaction is measured as absorbance.
• The color intensity is inversely proportional to the amount of FT3 in the sample.

Storage and stability:

• Store at 2-8℃.
• Seal and return unused reagents to 2-8℃, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.