Bovine Brucellosis

                     FOR RESEARCH USE ONLY

Drug Names

Bovine Brucellosis elisa test kit RUO 

Purpose

This kit allows for the determination of Brucellosis expression in Bovine serum, and other biological fluids.

Principle of the assay

The kit assay Brucellosis level in the sample, use Purified Brucellosis antibody to coat microtiter plate wells, make solid-phase antibody, then add Brucellosis to wells, Combined With Brucellosis, after washing and removing non-combinative antigen and other components ,then Combined Brucellosis antibody which with HRP labeled become antibody – antigen - enzyme- antibody complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge Brucellosis exist in the sample or not.

Test principle

Materials provided with the kit

Important notes

Specimen requirements Sealed bags

1. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

2. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Storage and stability

• Store at 2-8℃.

• Seal and return unused reagents to 2-8℃, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.