PPRV Ab ELISA Kit Veterinary

Principle of the Test

The PPRV antibody ELISA test kit use to detection of Peste des petits ruminants virus antibodies in the serum of sheep and goat . This kit use competitive ELISA method to pre-coated PPRV antigens on microplate wells. When testing, add diluted serum sample, after incubation, if there have PPRV antibody, it will combine with the pre-coated antigen, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample

Technical Parameters:

Test procedure

1.For every test, set 1 well for positive control and 2 wells for negative control;

2.Add sample diluent into each well, 30ul/well;

3.Add sample, negative control and positive control to its corresponding wells, 20ul/well. (Do not mix use the tips);

4.Adding PPRV Mab solution, 50ul/well, shake gentle to mix it evenly, cover it with Adhesive plate sealer, incubate at 37 ℃for 45 minutes; 5.Open the adhesive plate sealer,discard the liquid of the well,add diluted washing buffer to each well, 250ul/well, discard the liquid, repeat the above step for 4-6 times, at last flap to dry with the absorbent paper;

6.Adding Enzyme Conjugate, 100ul/well,Cover it with Adhesive plate sealer,incubate at 37℃ for 30 minutes;

7.Open the adhesive plate sealer, discard the liquid of the well, washing 4-6 times as step 5,remember at last flap to dry with the absorbent paper;

8.Add substrate, 100ul/well, mix it evenly then cover it with Adhesive plate sealer,incubate at 37 ℃ in dark for 10 minutes;

9.Add stop solution, 50ul/well to stop the reaction, measure the result in 10 minutes.

FAQ:

Q: What is the brand name of this test kit?

A: The brand name of this test kit is Biovantion.

Q: Where is this test kit manufactured?

A: This test kit is manufactured in China.

Q: Is this test kit certified?

A: Yes, this test kit is certified with ISO13485.

Q: What is the minimum order quantity for this product?

A: The minimum order quantity for this product is 10.

Q: What is the packaging details for this product?

A: The packaging details for this product is CARTON.

Q: What is the delivery time for this product?

A: The delivery time for this product is 7-15 days.

Q: What are the payment terms for this product?

A: The payment terms for this product is TT 100% PAYMENT.

Q: What is the supply ability for this product?

A: The supply ability for this product is 100000.

Q: Is the price of this product negotiable?

A: Yes, the price of this product is negotiable.