C-Peptide C-P ELISA Test Kit

Drug Names

Generic Name: C-Peptide C-P ELISA Test Kit

INTENDED USE

Detection Kit for C-peptide(C-P)(Enzyme-Linked ImmunoSorbent Assay , ELISA).It is used in quantitative tests for C-peptide(C-P)in human serum.

SUMMARY AND CLINICAL SIGNIFICANCE

The synthesis of insulin is the first synthesis of proinsulin in pancreatic beta cells, which are cleaved by enzymes, release of insulin and a peptide that connects insulin A and B chains in proinsulin, and therefore Known as C-peptide (C-P). Human C- peptide contains 31 amino acid residues, molecular weight of about 3600, no metabolic function. It is released with isotonic molecules, and the biological half-life of the C-peptide in the blood circulation is 2 to 5 times longer than that of the insulin, so that the level of the C-peptide is more stable to reflect the condition of insulin secretion and can be used to distinguish between endogenous insulin and insulin injections.

Product details

PRINCIPLE

C-P EIA kits are based on sandwich method. Coat micro well plates with one monoclonal antibody to form solid-phase antibody. Add patient serum and another monoclonal antibody labeled by HRP to wells to form antibody-C-P-HRP antibody complex. Add substrate and detect absorbent value (OD value). Calculate C-P content in patient serum through computer or plotting fitting concentration-absorbent value curve.

Important notes

• The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature then use, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
• washing buffer will Crystallization separation, it can be heated the water helps dissolve when

dilute . Washing does not affect the result.

• add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
• Please make specification curve when you assay, had better make duplicate well, if in the sample the testing material content is excessively high (The sample OD is bigger than the first standard well OD ),please use Sample dilution to dilute certain multiple (n times),then assay. Please multiply total Dilution Times when calculate(×n×5).
• Closure plate membrane only limits the disposable use, in order to avoid the overlapping pollution.
• The substrate please evade the light preservation.
• Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard .
• All samples, washing buffer and each kind of reject should according to infective material process.
• This reagent which different batch number component do not mix.
• If it’s different form English instruction, take English instruction as the standard.

Storage and stability

• Store at 2-8℃.

• Seal and return unused reagents to 2-8℃, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.