PCR Master Mix

Product Introduction Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product. Application • PCR amplification of DNA fragments as long as 5 kb

Product Description T4 gene 32 protein is a single-stranded DNA-binding protein encoded by the T4 phage gene 32, with a molecular weight of 33 KDa. It is essential for T4 phage DNA replication and repair, and plays a crucial structural role in the coordination and stabilization of transiently formed single-stranded DNA regions during the process of Rb69 phage DNA replication. The protein is also widely used to stabilize and label single-stranded regions for the observation of

Taq Mix Ⅱ Contents: 2× Taq Mix Ⅱ 1 ml Nuclease-free water 1 ml Store at -20°C For research use only. In total 2 vials. Description 2× Taq Mix Ⅱ is an upgraded product of Taq Mix, with better sensitivity and specificity. It’s a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. This

Description ARMS PCR Mix is a 2x PCR Mix solution developed for ARMS PCR, containing chemically modified hotstart Taq DNA polymerase, dNTPs, buffer and other essential components for PCR amplification (except templates and primers). When used, only templates and primers need to be added into the amplification system, greatly simplifying the operation process, shortening the operation time, and reducing pollution (less sampling times). The reaction system of ARMS PCR Mix is

Concentration: 5U/μl Contents: Hotstart KnTaq Polymerase (5U/ul) 50 μl 10× Taq Buffer (Mg2+ Plus) 1.25 ml Store at -20°C For research use only. In total 2 vials. Description Hotstart KnTaq Polymerase is an N-truncated Taq DNA polymerase with a stable hot-start system and no 5'-3' exonuclease activity. It is optimized for excellent differentiation of error extensions, and can be used for SNP analysis, genotyping and allele-specific PCR. Unit Definition One unit is defined as

Cat. No./Spec.Cat. NoK034-AK034-BK034-C50-μl reaction Nos.40 rxns200 rxns4,000 rxns DescriptionSuper TaqPlus Green PCR Mix is formulated with Invitrogen PlatinumII Taq Hot-Start DNA Polymerase and Platinum SuperFiII DNA Polymerase in a certain ratio, and has been verified by NGS to have similar fidelity performance to Platinum SuperFiII Green PCR Master Mix. And the amplification performance is stronger. Universal annealing temperatures can be used, which reduces the number

SuperⅡ Hotstart Taq Polymerase #P1211, 250U Concentration: 5U/μl Contents: SuperⅡ Hotstart Taq Polymerase (5U/ul) 50 μl 10× Hotstart Buffer (Mg2+ Plus) 1.25 ml Store at -20°C For research use only. In total 2 vials. Description SuperⅡ Hotstart Taq Polymerase is a dual-antibody modified hot-start polymerase with ultra-low nucleic acid background and high purity, which brings higher specificity by reducing non-specific products as the enzyme activity is temperature-dependent

Super HIFI DNA Polymerase For research use only Cat. No./Spec. Cat. No P1251 P1252 Spec. 100U 500U Description Super HIFI DNA Polymerase is a high-fidelity DNA polymerase. Its fidelity is approximately 50 times higher than that of regular Taq DNA polymerase, making it suitable for cloning and other applications that require high fidelity. Compared to other DNA polymerases, Super HIFI DNA Polymerase is robust, with short protocol time, tolerance to PCR inhibitors, and high

Product Introduction GDSlyo Hotstart Taq Polymerase is a hot-start Taq DNA Polymerase, which can not only better inhibit the non-specific reaction caused by non-specific annealing of primers or primer-dimer in the process of PCR system preparation and amplification, so that this product has excellent specificity, is more effective for the amplification of low-concentration templates, and is suitable for multiplex PCR amplification reactions, and this product has good system

Cat. No./Spec. Cat. No P1231 P1232 P1233 P1234 50-μl reaction Nos. 250U 1,000U 5,000U 50,000U Description CSM Taq Polymerase of GDSBio is a Cold Sensitive Mutant enzyme with low activity at room temperature and reactivation above 60°C. Provides excellent specificity, sensitivity, and yield. Amplify fragments up to 5 kb in length (simple template). The elongation rate is 2 min/kb (70-75°C, up to 20 s/kb for simple templates). It has 5’-3’ polymerase activity, but no 3’-5’

Cat. No./Spec.: P9131/500 U; P9132/5,000 U Concentration: 10 U/µL Description Taq Antibody is a monoclonal antibody against Taq DNA polymerase used in Hot Start PCR that binds to Taq to inhibit DNA polymerase activity and effectively inhibit nonspecific annealing of primers and nonspecific amplification induced by primer dimers. Anti-Taq antibodies denaturate during the initial DNA denaturation step of the PCR reaction, so no special anti-Taq antibody inactivation treatment

dUTP dUTP for PCR qPCR P9071 1ml #P9111, 1 ml Molecular biology grade Concentration: 100mM Formula: C9H12N2O14P3Na3 Molecular Weight: 534.1 Purity (HPLC area%) ≥99% Components Component P9111 dUTP (100mM) 1ml Store at -20°C Description dUTP (2’-deoxyuridine 5’-triphosphate) is supplied as a 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. Applications For use in PCR, real-time PCR and RT-PCR with Taq DNA Polymerase, cDNA synthesis and primer extension. Dongsheng

dGTP #P9101, 1 ml Molecular biology grade Concentration: 100mM Formula: C10H13N5O13P3Na3 Molecular Weight: 573.2 Components Component P9101 dGTP (100mM) 1ml Store at -20°C Description dGTP (2’-deoxyguanosine 5’-triphosphate) is supplied as a 100mM aqueous solution at pH 7.0. Applications For use in PCR, long PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing, DNA labeling. Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common

dCTP #P9091, 1 ml Molecular biology grade Concentration: 100mM Formula: C9H13N3O13P3Na3 Molecular Weight: 533.1 Components Component P9091 dCTP (100mM) 1ml Store at -20°C Description dCTP (2’-deoxythymidine 5’-triphosphate) is supplied as a 100mM aqueous solution at pH 7.0. Applications For use in PCR, long PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing, DNA labeling. Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common

dTTP #P9081, 1 ml Molecular biology grade Concentration: 100mM Formula: C10H14N2O14P3Na3 Molecular Weight: 548.1 Components Component P9081 dATP (100mM) 1ml Store at -20°C Description dTTP (2’-deoxythymidine 5’-triphosphate) is supplied as a 100mM aqueous solution at pH 7.0. Applications For use in PCR, long PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing, DNA labeling. Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common

dATP #P9071, 1 ml Concentration: 100mM Formula: C10H13N5O12P3Na3 Molecular Weight: 557.2 Components Component P9071 dATP (100mM) 1ml Store at -20°C Description dATP (2’-deoxyadenosine 5’-triphosphate) is supplied as a 100mM aqueous solution at pH 7.0. Applications For use in PCR, long PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing, DNA labeling. Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid

10× PCR Buffer (Mg2+ Plus) Component P5011 10× PCR Buffer (Mg2+ Plus) 1.25 ml × 4 Store at -20°C For research use only. In total 4 vials. Description 10× PCR Buffer (Mg2+ plus) is recommended for all PCR applications with Taq DNA Polymerase (both recombinant and native). Quality Control Amplification of a single-copy gene from human genomic DNA. Composition of the 10× PCR Buffer with Mg2+ 100mM Tris-HCl (pH8.8) 500mM KCl 1% Triton-X-100 16mM MgCl2 Dongsheng Biotech Co., Ltd

Pollution-proof 2X Multiplex PCR Master Mix with UDG Multiplex PCR Master Mix with UDG, 2X The Multiplex PCR Master Mix with UDG is a pollution-proof multiplex PCR premix containing all components (except primers and templates) required for multiplex PCR reactions. DUTP /UDG anti-pollution system is introduced in the Mix, and UDG enzyme can rapidly degrade the pollutants containing U at room temperature. Hotstart Taq DNA Polymerase's superior performance in combination with

The specificity Pollution - Proof 2X Multiplex DNA RNA PCR Master Mix With UDG PM2001 PM2002 PM2003 Pollution-proof 2X Multiplex PCR Master Mix with UDG Multiplex PCR Master Mix with UDG, 2X The Multiplex PCR Master Mix with UDG is a pollution-proof multiplex PCR premix containing all components (except primers and templates) required for multiplex PCR reactions. DUTP /UDG anti-pollution system is introduced in the Mix, and UDG enzyme can rapidly degrade the pollutants

Ultra High Fidelity Super HIFI PCR Master Mix Pass Activity High-specificity amplification & multiplex PCR P2111 Super HIFI PCR Master Mix Contents: Component P2111 (100 rxns) P2112 (1000 rxns) P2113 (5000 rxns) 2X Super HIFI PCR Master Mix 1 ml × 1 1 ml × 10 10 ml × 5 GC Enhancer 0.25 ml × 1 1 ml × 2 10 ml × 1 Store at -20°C Description Super HIFI PCR Master Mix is a PCR premix containing hotstart proofreading DNA polymerase and contains the various components (except