PCR Master Mix

HSTM Kit #P3082, 5 ml Contents: 2× HSTM Reaction Mix 1 ml × 5 HSTM Taq DNA Polymerase (2.5U/μl) 160 μl Store at -20°C For research use only. In total 6 vials. Description HSTM Kit contains HSTM Taq DNA Polymerase and a premixed, ready-to-use HSTM Reaction Mix, which contains dNTPs, Mg2+ and other components at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. The reaction mix

HSTM Taq DNA Polymerase Description HSTM Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. HSTM Taq DNA Polymerase can amplify DNA target up to 5 kb. The elongation velocity is 2kb/min. It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product. All components of the HSTM PCR Buffer are at optimal concentration

OptimusTM Hotstart Taq Mix Optimus Hotstart Taq Mix PCR Master Mix P2042 5ml #P2042, 5 ml Contents: 2× OptimusTM Hotstart Taq Mix 1 ml × 5 Nuclease-free water 1 ml × 5 Store at -20°C For research use only. In total 2 vials. Description 2× OptimusTM Hotstart Taq Mix is a premixed, ready-to-use solution containing Hotstart Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR,

OptimusTM Hotstart Taq DNA Polymerase #P1042, 1,000U Concentration: 5U/μl Contents: OptimusTM Hotstart Taq DNA Polymerase (5U/ul) 200 μl 10× Hotstart Buffer (Mg2+ Plus) 1.25 ml × 2 6× Loading Buffer 1 ml Store at -20°C For research use only. In total 4 vials. Description OptimusTM Hotstart Taq DNA Polymerase is a hot-start polymerase with chemical modification, which brings higher specificity by reducing non-specific products as the enzyme activity is temperature-dependent

HS Hotstart Taq DNA Polymerase For research use only HS Hotstart Taq DNA Polymerase pcr reagent high specificity P1091 500U Components Component P1091 (500 U) 10 × HS Hotstart Taq Buffer (Mg2+ plus) 1 ml × 2 dNTP Mix (10 mM each) 400 μl HS Hotstart Taq DNA Polymerase (5 U/μl) 100 μl Storage This reagent should be kept at -30 ~ -15°C. Description HS Hotstart Taq DNA Polymerase is a hot-start Taq DNA polymerase produced by mixing Taq antibody with Taq DNA polymerase in optimum

Water (nuclease-free, molecular biology grade) Contents: Water (nuclease-free, molecular biology grade) Cat. No. Size P9021 1 ml × 5 P9022 100 ml P9023 500 ml Store at -20~30°C. For research use only. Description The Water, nuclease-free, is deionized and 0.22 µm membrane-filtered. It is suitable for use in all molecular biology applications. Feature Free of endodeoxyribonucleases, exodeoxyribonucleases, ribonucleases and phosphatases. Quality Control The absence of

PCR Sample Preparation Solution PCR Sample Preparation Solution P9051 P9052 #P9051, 50 preps #P9052, 250 preps Components Content P9051 P9052 Extracion Solution 10 ml 40 ml Neutralization Solution 1 ml 5 ml Store at RT For research use only. Quality Control The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests. Protocol 1. Sample Preparation a. For tissue Add 3-5mg of sample tissue to a 1.5ml microcentrifuge

Heat Labile UDG For research use only Heat Labile UDG Highly effective anti-contamination enzyme R5001 Components Component R5001 (500 U) R5002 (100 U) Heat Labile UDG (1 U/μl) 500 μl 100 μl Storage This reagent should be kept at -20°C. Description Heat Labile UDG is purified from the expression product of an recombinant E. coli strain containing the UDG gene cloned from cold-adaptive marine bacteria (psychrophilic Marine bacterium). UDG (Uracil-DNA Glycosylase) catalyzes the

HSTM Mix #P2081, 1 ml HS Mix PCR Master Mix P2081/1ml Contents: 2× HSTM Mix 1 ml Nuclease-free water 1 ml Store at -20°C For research use only. In total 2 vials. Description 2× HSTM Mix is a premixed, ready-to-use solution containing HSTM Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. HSTM Mix contributes to highly reproducib

OptimusTM Hotstart Taq Mix #P2041, 1 ml Contents: 2× OptimusTM Hotstart Taq Mix 1 ml Nuclease-free water 1 ml Store at -20°C For research use only. In total 2 vials. Description 2× OptimusTM Hotstart Taq Mix is a premixed, ready-to-use solution containing Hotstart Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. This premixed

Heat Labile UDG For research use only Heat Labile UDG Highly Effective Anti-Contamination Enzyme R5001R5002 Components Component R5001 (500 U) R5002 (100 U) Heat Labile UDG (1 U/μl) 500 μl 100 μl Storage This reagent should be kept at -20°C. Description Heat Labile UDG is purified from the expression product of an recombinant E. coli strain containing the UDG gene cloned from cold-adaptive marine bacteria (psychrophilic Marine bacterium). UDG (Uracil-DNA Glycosylase)

FSTM Taq Mix Direct for Blood #P2071a, 1 ml Contents: 2× FSTM Mix B 1 ml Nuclease-free water 1 ml Store at -20°C For research use only. In total 2 vials. Description 2× FSTM Taq Mix Direct for Blood (FSTM Mix B) is a premixed, ready-to-use solution containing FSTM Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. FSTM Mix B is specific for whole blood amplification. It contributes to fast,

FSTM Taq Mix Direct for Tissue #P2071b, 1 ml Contents: 2× FSTM Mix T 1 ml Extraction Solution 10 ml Neutralization Solution 1 ml Nuclease-free water 1 ml Store at -20°C For research use only. In total 4 vials. Description 2× FSTM Taq Mix Direct for Tissue (FSTM Mix T) is a premixed, ready-to-use solution containing FSTM Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. FSTM Mix T is specific for

FSTM Taq Mix Direct for Blood #P2072a, 5 ml Contents: 2× FSTM Mix B 1 ml × 5 Nuclease-free water 1 ml × 5 Store at -20°C For research use only. In total 10 vials. Description 2× FSTM Taq Mix Direct for Blood (FSTM Mix B) is a premixed, ready-to-use solution containing FSTM Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. FSTM Mix B is specific for whole blood amplification. It contributes to

FSTM Taq Mix Direct for Tissue #P2072b, 5 ml Contents: 2× FSTM Mix T 1 ml × 5 Extraction Solution 40 ml Neutralization Solution 5 ml Nuclease-free water 1 ml × 5 Store at -20°C For research use only. In total 12 vials. Description 2× FSTM Taq Mix Direct for Tissue (FSTM Mix T) is a premixed, ready-to-use solution containing FSTM Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. FSTM Mix T is

dNTP Set #P9061, 250 μl × 4 molecular biology grade Components of the Set dATP (100mM) 250 μl dTTP (100mM) 250 μl dCTP (100mM) 250 μl dGTP (100mM) 250 μl Store at -20°C For research use only. In total 4 vials. Description The set consists of 100mM aqueous solutions of dATP, dTTP, dCTP and dGTP each supplied in a separate vial. Since the nucleotides are provided separately, the dNTP Set offers maximum flexibility in preparation of reaction mixes for different applications.

dNTP Mix Concentration: 10mM each Components Component P9012 dNTP Mix 0.5ml Storage Store at -20°C Description dNTP Mix is aqueous solutions at pH 7.0 containing dATP, dCTP, dGTP and dTTP, each at a final concentration of 10mM. dNTP Mix is ready-to-use solutions designed to save time and to provide higher reproducibility in PCR and other applications. The Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set-up errors. Applications

dNTP Mix Concentration: 10mM each Components Component P9013 dNTP Mix 1ml Storage Store at -20°C Description dNTP Mix is aqueous solutions at pH 7.0 containing dATP, dCTP, dGTP and dTTP, each at a final concentration of 10mM. dNTP Mix is ready-to-use solutions designed to save time and to provide higher reproducibility in PCR and other applications. The Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set-up errors. Applications For

dNTP Mix Concentration: 2.5mM each Components Component P9011 dNTP Mix 1ml Storage Store at -20°C Description dNTP Mix is aqueous solutions at pH 7.0 containing dATP, dCTP, dGTP and dTTP, each at a final concentration of 2.5mM. dNTP Mix is ready-to-use solutions designed to save time and to provide higher reproducibility in PCR and other applications. The Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set-up errors. Applications

PCR Enhancer #P9041, 500 µl Store at -20°C For research use only. In total 1 vials. Description PCR Enhancer System is used in conjunction with DNA polymerases to optimize PCR of problematic and/or GC-rich templates. The system offers higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved themostability DNA polymerases. Applications • Complex template with GC-rich • High efficiency, quality and yield Usage Add 2 – 8 μl PCR