Quick Accurate 100T 500T Reverse Transcriptase Kit RT PCR EasyTM II Kit
Quick and accurate RT-PCR EasyTM II(Two Step) Reverse Transcriptase Kit
Product Descprition
RT-PCR EasyTM II(Two Step) contains all the reagents for two-step RT-PCR, combining RT reaction and PCR reaction in the same kit, providing optimized RT Mix and PCR Mix. The unique RT Mix can synthesize high-quality first-strand cDNA easily and efficiently. 2× PCR EasyTM Mix Plus has high-efficiency and specific amplification. The organic combination of the two makes the detection of the target gene more sensitive.
Product components:
| RT-PCR EasyTM II(Two Step) | ||
| Kit contents | RT-02021 | RT-02022 |
| 100T (20μl system) | 500T (20μl system) | |
| 2× RT EasyTM Mix | 1ml | 1.7ml×3 |
| 2× PCR EasyTM Mix Plus | 1ml | 1.7ml×3 |
| Random Primer (50μM) | 200μl | 1ml |
| Oligo(dT)18 Primer (50μM) | 200μl | 1ml |
| RNase-Free ddH2O | 1.7ml | 1.7ml |
| Instruction Manual | 1 piece | 1 piece |
Features&advantages:
- Real-time quantitative analysis of RNA can be carried out quickly and accurately.
- The kit uses a unique Foregene reverse transcription reagent and Foregene HotStar Taq DNA Polymerase combined with a unique reaction system to effectively improve the amplification efficiency and specificity of the reaction.
- The optimized reaction system makes the reaction have higher detection sensitivity, stronger thermal stability, and better tolerance.
Kit component information
- 2× RT EasyTM Mix:Foregene Reverse Transcriptase, RNase Inhibitor, dNTPs, reaction buffer, optimizer and stabilizer, etc.
- 2× PCR EasyTM Mix Plus :Foregene Taq DNA Polymerase, dNTPs, Mg2+,reaction buffer, optimizer and stabilizer.
Product quality control:
In accordance with FOREGENE’s Total Quality Management System (FOREGENE’s Total Quality Management System), each batch of RT-PCR EasyTM II(Two Step) kits is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.
Form 1:RT-PCR system preparation
| RT-PCR system additions | Amount |
| 2× RT EasyTM Mix | 10μl |
| Random Primer(50μM) | 1μl |
| or Oligo(dT)18 Primer(50μM) | 1μl |
| or Specific Primer(2μM) | 1μl |
| Template(RNA) |
Xμl (Total RNA:<5μg / mRNA:<0.5μg) |
| RNase-FreeddH2O | (9-X)μl |
| Total Volume | 20μl |
Storage and Shelf life:
The kit should be stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.
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