PCR Hero (With Dye) Ultra-Efficient PCR Reaction Premix System PCR Kits Suitable For A Variety PCR Templates

Description:

This kit includes Foregene's unique new generation of Taq—Foregene Taq Hero, optimized dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The 2× PCR HeroTM Mix (dye) system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.

Specifications:

5ml, 10ml, 40ml

Kit(2× PCR HeroTM Mix) components:

Features&advantages:

-Higher fidelity: 6 times that of ordinary Taq enzyme;

-Faster amplification speed : the amplification speed is 5-10sec/kb, which is 6-12 times that of ordinary Taq enzyme;

-More template adaptability: It can efficiently amplify various complex DNA templates with high GC content;

-Higher amplification efficiency: lower amplification cycles than ordinary Taq enzyme;

-Environmental tolerance is stronger: placed at 37°C for a week, maintaining more than 90% activity;

-It has 5’→3’ DNA polymerase activity and 5’→3’ exonuclease activity, without 3’→5’ exonuclease activity.

The kit is pre-mixed with Electrophoresis Loading Buffer, after PCR is completed, the sample can be directly loaded for electrophoresis; the operation is simple and easy.

Practical examples

1.Reaction system 

2.Reaction conditions

1*: If using plasmid as template, the extension speed is 5sec/kb, we recommend using 10sec/kb; if using genomic DNA as the template, the extension speed is 10sec/kb, we recommend using 20sec/kb. If you use a highly complex template that is difficult to amplify, it is recommended to increase the extension time by 10sec/kb on the original basis.

Note: For 10µl and 20µl systems, if the PCR machine does not have a thermal cover, add an equal volume of mineral oil. The PCR reaction conditions are based on the specific conditions of the template, primer set, and the GC content and length of the primers to design the best reaction conditions, including annealing temperature, extension time, etc.

Kit application:

PCR amplification of DNA fragments, DNA labeling, DNA sequencing,PCR plus A tail

Storage and Shelf life:

It can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.