EasyTM (One Step) Taqman RT QPCR Kit For Molecular Biology Applications

Intended use

The RT-qPCR EasyTM (One Step)-Taqman Kit is intended for molecular biology applications.

Introduction

The RT-qPCR EasyTM (One Step)-Taqman Kit provides rapid real-time quantification of RNA targets. The kit contains optimized components that allow both reverse transcription and PCR amplification to take place. With this kit, all steps can be performed in a single tube.

Kit contents

Shipping and storage conditions

When stored under these conditions and handled correctly, the kit can be kept one year without showing any reduction in performance.

• The kit is shipped on ice pack or dry ice.

• The kit should be stored at -20 ± 5℃.

• Avoid repeated freeze-thaw cycles.

Protocol

Important notes before starting:

• Set up all reactions on ice and mix the solutions completely before use.

• This kit requires gene-specific primers for PCR.

• An RNase-free environment should be maintained during reaction setup.

Procedure:

• Prepare the PCR Reaction Mix

  • Combine the following components for the number of reactions required, plus 10% overage.

1*: A final primer concentration of 400 nM is optimal for most reactions.

2*: Final ROX concentration for different instruments:

• Mix the components gently but thoroughly and dispense appropriate volumes into PCR tubes.

• Run the reaction:

  • Thermal cycler conditions:

or

1*: If satisfactory results are not obtained using 42°C, the reaction temperature may be increased up to 50°C.

2*: The extension rate of Foregene HotStar Taq DNA Polymerase is 2 kb/min in PCR.

After setting, save the file and run the reaction program.