Malachite Green(MG) ELISA Test Kit bran mash, eggs, honey, meat/liver/kidney, milk and urine

Malachite Green(MG) ELISA Test Kit

            CH86108

Product Description

                Malachite Green plate kit is a competitive ELISA for the quantitative analysis of Malachite green in fish/shrimp.

The unique features of the kit are:

• High, reproducible recovery rates
• Converts Leucomalachite Green (LMG) to Malachite Green before detection. Chromatic Malachite Green can be rapidly bio-converted into colorless LMG in fish. A unique oxidant solution in the kit converts LMG to the Malachite Green form detected by the kit.
• 96-well high-throughput quantitative detection method. The detection limit is 0.1ng/g or ppb for fish/shrimp samples.
• Final samples can be directly used for liquid chromatography confirmation.

Procedure Overview

              Malachite Green plate kit is a competitive enzyme -labeled immunoassay. Malachite Green is extracted from a sample by blending or shaking with extraction solvent. The diluted Malachite Green sample extract and calibrators are pipetted into the test wells followed by Malachite Green antibody into the test wells to initiate the reaction. After this 30 minute incubation the wells are washed with laboratory grade water. Add Malachite Green HRP conjugate for 30 minutes. Following a second wash after the incubation, a clear substrate is added to the wells and any bound enzyme conjugate causes the conversion to a blue color. After the 15 minute incubation, the reaction is stopped and the amount of color in each well is read . The color of the unknown samples is compared is compared to the color of the calibrators and the Malachite Green concentration of the samples is derived.

Kit Contents, Storage and Shelf Life

            Malachite Green ELISA Test Kit has the Malachite greenacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.