Tetrodotoxin TTX ELISA Test Kit 1.5ppb High Sensitivity And Simple Operation

Price Negotiable

Price: Negotiable

MOQ: 1kits

Delivery Time: 5-8 work day

Brand: Chenhui

Product Description

Tetrodotoxin ELISA Kit

CH98016

Tetrodotoxin ELISA Kit provides a competitive enzyme immunoassay for the quantitative analysis of Tetrodotoxin in Pufferfish.Up to 96 tests or assaying about 42 samples in duplicate( assuming 12 wells for standards).

1.Sample Preparation

Reagent Preparation

Extraction-solvent

The formulation of Extraction-solvent is as follow: methanol:distilled water:glacial acetic acid=400:599:1; for exemple, methanol 40mL+distilled water 59.9mL +glacial acetic acid 0.1mL

1×sample-diluent

Mix 1 volume of 4×sample-diluent with 3 volumes of distilled water.

Pufferfish(muscle, liver, skin)

Homogenize a reasonable amount of sample with a suitable mixer.
Weigh out 2g of the homogenize sample into a suitable vial and mix thoroughly with 10mL of Extraction-solvent.
Keep the vial staying in a 90℃ water bath for 10 minutes and during this procedure, shake the vial for 3 times .
Rinse the vial for 1minute with tap water to cool down the sample and then add 1mL of hexane, vortex for 30 seconds;
Centrifuge the sample at 4,000×g for 5 minutes, piptte 100μL of the interlayer liquid and add 300μL of 1×sample-diluent
Use 50μL of the sample solution per well for the assay.

Note: Dilution Factor :20