The unique features of the kit are:

1.Rapid (＜30min), high recovery (≥80%), and cost-effective extraction methods .

2.High sensitivity (1.5ug/kg or ppb) and low detection limit (30 ug/kg orppb) .

3.A quick ELISA assay (less than 1.5 hours regardless of number of samples).

4.High reproducibility.

• When compared to quantification by LC/MS or GC/MS, it is not uncommon for immunoassays to report higher analyte concentrations. While LC/MS or GC/MS methods typically measure only a single compound, antibodies used in immunoassays not only recognize the target molecule, but also structurally related molecules, including biologically relevant metabolites. So it is the responsibility of the client to understand the limits of both assays system and to interpret their data accordingly.
• Any variation in operating procedure, pipetting technique, washing technique, incubating time or temperature etc, can cause variation in result.
• Do not use the kit beyond the expiration date.
• Do not mix or substitute reagents with those from other lots or sources.
• Water used to prepare all ELISA reagents and buffers must be deionized or distilled water.
• Tetrodotoxin standards contain tetrodotoxin, a neurotoxin, so please be careful in use.

1.Microtiter plate reader (450 nm)

2.Incubator

3.Tissue Mixer

4.Thermostatic Waterbath

5.Vortex mixer

6. 10, 200 ,1000 and 5000 μLpipettes

7.Multi-channel pipette: 50-300 mL (Optional)

8.Methanol, hexane and glacial acetic acid ( purity ＞98%)