Product Description

          Furazolidone (AOZ) ELISA Test Kit provides a competitive enzyme mmunoassay for the quantitative analysis of furazolidone in feed, fish, shrimp, meat (beef, chicken and pork), eggs, honey, milk, serum and urine.

The unique features of the kit are:

• Rapid, high recovery (75 - 105%), and cost-effective extraction methods for various samples.
• High sensitivity (0.02 ng/g or ppb) and low detection limit (0.04 ng/g or ppb) for various samples.
• High reproducibility.
• A quick ELISA assay (less than 1 hour regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The AOZ antibody has been coated in the plate wells. During the analysis, sample is added along with the AOZ-horseradish peroxidase (AOZ-HRP) conjugate. If the AOZ residue is present in the sample, it will compete for the AOZ antibody, thereby preventing the AOZ-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the AOZ residue concentration in the sample.