Dibutylphthalate(DBP) ELISA Test Kit
CH580912
This product can adopt single-point calibration, that is, it can achieve quantitative analysis by pointing at one standard substance, and can test up to 47 samples at most. When single-point calibration is adopted, for data analysis, please call to request a dedicated Excel data analysis software
Sample pretreatment
(animal and vegetable edible oils, such as rapeseed oil, soybean oil, peanut oil, corn oil, blended oil, etc.)
| |
|
The reagents and vessels required but not provided are as follows:
Dimethyl sulfoxide, analytical pure
(2) Deionized water
(3)5mL glass centrifuge tube, 2mL glass vial
Edible oil for animals and plants
Weigh 1.5±0.01g of the oil sample into a 5mL glass centrifuge tube, add 1.5mL of dimethyl sulfoxide and shake for 3 minutes;
2) Centrifuge at 4000rpm for 1 minute;
3) Discard the upper liquid layer, take 1mL of the lower layer and add it to the purification tube, then add 0.2mL of DBP purification reagent A and shake for 1 minute;
4) Centrifuge at 4000 RPM for 1 minute;
5) Take 0.1mL of the supernatant and add it to 0.9mL of sample diluent, then vortex for 15 seconds;
Sample dilution factor: 10
2. Operating steps
Before the experiment, please take the test kit out of the refrigerator at 2-8℃ and balance it at an indoor temperature of 20-28℃ for more than 1 hour!
Reagent preparation
Washing solution (1×) : Take 1 volume of 20× concentrated washing solution, add 19 volumes of deionized water or distilled water, mix well, and set aside. It can be prepared as needed
1) Take out an appropriate amount of balanced strips and put the rest back into the aluminum film bag.
2) Add 150μL of DBP activation reagent to the microwells; Tap the board frame gently;
3) Cover the plate, let it stand at 20-28℃ for 5 minutes, then pour it out and pat out the liquid in the holes as dry as possible on a plate cloth.
4) Add 50μL of the standard/sample to the corresponding microwells in sequence; Add 50μL of enzyme conjugate and 50μL of antibody to all microwells; Tap the board frame gently;
5) Cover the plate and incubate at 20-28℃ for 20 minutes. Pour out the liquid in the micropores;
6) Fill the micropores with the washing solution, soak for 15 seconds, then pour it out. Repeat this process 4 times for a total of 5 times. And pat out the liquid in the hole as dry as possible on the racket cloth;
7) Add 100μL of substrate to the microwells, gently tap the plate rack, cover the plate tape, and incubate at 20-28 ° C for 10 minutes.
8) Remove the plate patch, do not pour out the liquid, directly add 100μL of stop solution to the microwells, gently tap the plate frame, and take the reading at a wavelength of 450/630nm.
|
